Effects of prenatal and perinatal probiotic supplementation on the development of experimental periodontitis in pregnant mice and their offspring: microbiome analysis

Abstract

The objective of this research will be to analyze how maternal prenatal and perinatal probiotic (PROB) supplementation affects the microbiome during the development of experimental periodontitis (PD) in the offspring of mice. Forty-eight pregnant mice will be used, divided into 4 groups (n = 12): CM (animals without PD and not treated with probiotics); PDM (animals with PD and not treated with probiotics); CMP (animals without PD and treated with probiotics) and PDMP (animals with PD and treated with probiotics). The offspring of these groups will be divided into 2 subgroups: P1 (without induction of PD; n = 6) and P2 (with induction of PD; n = 6). After mating and confirmation of pregnancy, animals in the PDM and PDMP groups will receive gavages with Porphyromonas gingivalis w83 (5x109 colony forming units (CFU) / mL) for 37 days. In offspring animals (P2 subgroups), PD will be induced following the same protocol adopted for the PDM and PDMP groups, with gavages performed for 21 days. Maternal animals in the CMP and PDMP groups will receive 1 x 109 CFU/mL of Bifidobacterium animalis subsp. lactis HN019 administered in water for 56 days, beginning 14 days before the animals were mated. Animals in the CM, CMP, PDM and PDMP groups will be euthanized on the 63rd. day of the experiment. Animals from subgroups P1 and P2 will be euthanized 63 days after birth. The oral and fecal microbiome will be evaluated, in addition to the amniotic fluid of the maternal and offspring groups by 16S rRNA metagenomic sequencing. The data obtained will be submitted to statistical analysis (p <0.05). (AU)