Expression control of immuno-inflammatory markers in conventional glass ionomer cement incorporated from titanium nanotubes on odontoblastic cells

Abstract

Titanium dioxide nanotubes (n-TiO2) has been suggested as promising material for different applications. In order to determine the impact of the addition of n-TiO2 on the Physico-chemical properties of glass ionomer cement (GIC), our research group performed a series of in vitro studies (ARP FAPESP #2016/13786-0; 2019/14078-8). In general, the results show that the presence of n-TiO2 into GIC composition produces an improvement in the material's wear resistance and greater hardness without altering the morphology of fibroblasts cultured on the GIC prepared with different concentrations of nanotubes. Despite the significant advance in this area, it is not known if the presence of TiO2 nanotubes in the GIC composition can modulate the expression pattern of inflammatory cytokines. So, the present study aims to determine if the presence of n-TiO2 in GIC composition modifies the expression pattern of inflammatory cytokines in the culture of fibroblasts (NIH / 3T3). Different concentrations of n-TiO2 (3%, 5%, 7% in weight) synthesized by the alkaline method (E20 nm) will be incorporated into the GIC [Ketac Molar EasyMix - (KM)]. Odontoblasts will be cultured on GIC discs with and without nanotubes for 18 and 30 h and the following tests will be performed: 1. Cell proliferation assay (Trypan Blue and MTT), 2. Confocal Microscopy (Confocal), 3. Determination of cytokine levels (IL-1², IL-6, IL-10, VEGF, and TNF) using multiplex technology (n=6; 12 and 18 h), 4. Transcript levels for biomineralization markers (DMP1 and DSPP) (n=6, 1, 3, and 5 days). After the results are collected, they will be submitted to statistical analysis, considering the 5% level of significance.(AU)