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Effect of the treatment of soybean seeds with micronutrients in association with ultrasound assessed by multispectral imaging

Grant number: 21/01329-2
Support type:Scholarships in Brazil - Master
Effective date (Start): September 01, 2021
Effective date (End): June 30, 2022
Field of knowledge:Agronomical Sciences - Agronomy - Crop Science
Principal researcher:Clíssia Barboza da Silva
Grantee:Érica Souza Gomes
Home Institution: Centro de Energia Nuclear na Agricultura (CENA). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Associated research grant:17/15220-7 - Non-destructive image analysis methods for seed quality evaluation, AP.JP

Abstract

The optical technology of multispectral imaging integrated with artificial intelligence has enabled the detection of physical, physiological and chemical changes of seeds in an objective and non-invasive way. The treatment of seeds with micronutrients such as zinc, boron, copper and manganese has revealed promising effects, improving the physiological potential of seeds and initial establishment of seedlings from different crops. When associated with the ultrasound technique, the absorption of these elements by seeds can be influenced by the phenomenon of acoustic cavitation, which favors the fluidity of the cell wall promoting acceleration in the kinetics of intercellular transport. The main objective of this proposal is to study the effects of treatments with zinc, boron, copper and manganese in association with the ultrasound technique on the physiological potential of soybean seeds using multispectral imaging technologies, allowing to detect early signs that are not perceptible to the human eye. The optical images will be compared to the germination and vigor tests (first count in the germination test, traditional accelerated aging and saturated-salt accelerated aging - SSAA, seedling emergence and emergence speed index - ISE). Furthermore, micronutrients applied to the seeds with or without ultrasound will be mapped using X-ray fluorescence microscopy for a better understanding of the absorption kinetics. It is expected to find optical imaging patterns for non-invasive assessment of soybean seeds treated with micronutrients integrating ultrasound, opening new perspectives for the development of strategies related to biofortification, and offering alternatives that can contribute to better establishment of seedlings in the field. (AU)

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