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Application of enzymatic treatments and lignin nanoparticles in the development of a customized nanocellulose for the manufacture of active and sustainable packaging

Grant number: 21/04324-1
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): August 01, 2021
Effective date (End): July 31, 2025
Field of knowledge:Biological Sciences - Biochemistry - Enzymology
Principal Investigator:Valdeir Arantes
Grantee:Bruno Las Casas Chaves
Host Institution: Escola de Engenharia de Lorena (EEL). Universidade de São Paulo (USP). Lorena , SP, Brazil


Due to several aspects such as high performance, biodegradability, and the growing market perspectives, nanocellulose emerges as a material with potential to not only replace synthetic polymers, but also to develop green and active food packaging. Thus, this project aims to develop and analyse specific enzymatic treatments in order to produce nanocellulose with customized properties - such as barrier properties, transparency, antioxidant and antimicrobial activities - for food packaging applications. Nonetheless, the greatest challenge faced by customization is to reach an equilibrium between the properties once there is a trade-off between them. Therefore, this project aims to examine specific enzymatic pre- and pos-treatments, and the incorporation of lignin nanoparticles (LNP) to produce a customized cellulose nanofibrils (CNF) for further development of green and active packaging. Customization process will be divided into three stages and for each stage different conditions will be assayed: 1) endoglucanase pre-treatment; 2) xylanase post-treatment; 3) incorporation of LNPs. In each stage, CNFs will be selected regarding desirable properties for food packaging application. Finally, studies about production, characterization, biodegradability, and validation of the produced packaging will also be performed. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
LAS-CASAS, BRUNO; DIAS, ISABELLA K. R.; YUPANQUI-MENDOZA, SERGIO LUIS; PEREIRA, BARBARA; COSTA, GUILHERME R.; ROJAS, ORLANDO J.; ARANTES, VALDEIR. The emergence of hybrid cellulose nanomaterials as promising biomaterials. International Journal of Biological Macromolecules, v. 250, p. 27-pg., . (21/04324-1, 21/07023-2)
LAS-CASAS, BRUNO; ARANTES, VALDEIR. Endoglucanase pretreatment aids in isolating tailored-cellulose nanofibrils combining energy saving and high-performance packaging. International Journal of Biological Macromolecules, v. 242, p. 15-pg., . (21/04324-1, 21/07023-2)

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