Studies on structure & activity of RuII / arene / mercaptoligants complexes against cancer

Abstract

The present project aim the synthesis and characterizing ruthenium II semi-sandwich complexes containing binders of biological interest, as new candidates for metallopharmaceuticals, being an integral part of the thematic project "X-Ray Diffraction as a Tool in the Development of Potential Drugs" (2017 / 15850-0). In the synthesis stage, it is intended to perform a "screening" to evaluate the viability of coordination of mercaptoligantes, which are derived from mercaptopiridines and mercaptopirimidines, with ruthenium II precursors of general formula [Ru (h6-p-cimene) Cl2]2 and [Ru (h6-p-cymene) Cl2 (P)] where P refers to monophosphines. The synthesized compounds will be characterized by techniques such as 1H, 13C and 31P {1H} nuclear magnetic resonance spectroscopy, cyclic and differential pulse voltammetry, conductometry, absorption spectroscopy in the ultraviolet / visible / infrared region and elementary analysis. In addition, he will dedicate himself to the characterization of the compounds in the solid state, mainly by the diffraction of X-rays by single crystals (equipment operation, data collection and refinement), of the structural and crystallographic properties exhibited by these complexes and others belonging to the thematic project, and from the structural information elucidate some physicochemical properties of the same. Subsequently, it is intended to study the possible interactions of these complexes with biomolecules such as: DNA and HSA. The influence of the compounds on the cell cycle, apoptosis by annexing V assay, quantification of reactive oxygen species, as well as determination of proteins related to apoptosis will also be evaluated. The results obtained will be compared with each other and with data from the literature, seeking a better understanding of the molecular regions responsible for their biological, chemical and structural properties, in order to explore how the compounds can interact with them and elucidate their mechanisms of action. (AU)