Association of rs4284742 genetic variation in the SIGLEC5 gene with periodontitis in the Brazilian population

Abstract

Aggressive periodontitis has a great impact on patients' quality of life, as it presents an early onset, rapid and severe progression, poor response to therapeutic approaches and a high rate of tooth loss. The susceptibility to this disease is determined by a complex relationship between periodontopathogens (bacterial challenge) and the host's response, this relationship being regulated by genetic mechanisms. Recent large-scale studies have reported the association between rs4284742 genetic polymorphism (SIGLEC5) A> G with aggressive and chronic periodontitis in European and Asian populations. Thus, the aim of the present study will be to investigate the frequency of the rs4284742 variation in the SIGLEC5 gene in groups with rapid progression (Grade C) and slow or moderate progression (Grade A and B) and in periodontal healthy individuals, to observe an association between this variation and periodontitis in the Brazilian population, in addition to associating these results with the results obtained for the American population. For that, the genomic DNA will be extracted and purified from oral epithelial cells obtained by mouth washing with 3% dextrose. Soon after, the genotypes will be identified through real-time PCR using the Taqman® probe system. The distribution of the genotypes for the polymorphism will be evaluated for deviation from the Hardy-Weinberg balance and the differences in the genotypic and allelic frequencies between the groups will be assessed by the chi-square test. All statistical analyzes will be performed considering the 5% significance level. The research will be carried out in the area of Periodontics at FOP/UNICAMP.