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Application of bioluminescence for cell tracking and inflammatory processes: in vitro and in vivo study

Grant number: 20/07649-6
Support type:Scholarships in Brazil - Post-Doctorate
Effective date (Start): May 01, 2021
Effective date (End): April 30, 2023
Field of knowledge:Engineering - Biomedical Engineering - Bioengineering
Principal researcher:Eliana Aparecida de Rezende Duek
Grantee:Vanessa Rezende Bevilaqua
Home Institution: Faculdade de Ciências Médicas e da Saúde (FCMS). Pontifícia Universidade Católica de São Paulo (PUC-SP). Sorocaba , SP, Brazil
Associated research grant:10/05426-8 - Arthropod bioluminescence: biological diversity in Brazilian biomes, biochemical origin, structural/functional evolution of luciferases, molecular differentiation of lanterns, biotechnological, environmental and educational applications, AP.TEM

Abstract

The bioluminescent reporter genes that encode luciferases are among the most sensitive and frequently used to monitor non-invasively cellular processes, in real time. Unlike fluorescent reporter genes such as GFP, which have disadvantages such as autoabsorption of irradiated light, autofluorescence and phototoxicity, bioluminescent reporter genes have the advantage of producing their own light through the expression of luciferase enzymes that catalyze the bioluminescent oxidation of substrates known as luciferins. Reporter genes are employed for a variety of purposes, including Bioluminescence Imaging (BLI) of processes such as cell differentiation and growth and testing on animal models that have helped the pharmaceutical industry test drugs, reducing statistical bias and sequential animal sacrifice. The use of BLI has also been used to screen cells of the immune system. Bioluminescent reporter genes have been used to accompany tissue regeneration and repair processes by monitoring mesenchymal stem cells administered in injured areas that have been shown to assist in the process. In order to advance the studies related to skin regeneration, in this project we plan to produce bioluminescent cell lines from mesenchymal stem cells, using beetle luciferase reporter genes, to monitor mammalian cells in regeneration and tissue growth processes in biomaterials, and associated inflammation processes. (AU)

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