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Influence of a catalytic primer on the aesthetic effectiveness, degradation kinetics and cytotoxicity of bleaching gels

Grant number: 20/08882-6
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): April 01, 2021
Effective date (End): February 28, 2022
Field of knowledge:Health Sciences - Dentistry - Dental Materials
Principal Investigator:Carlos Alberto de Souza Costa
Grantee:Marlon Ferreira Dias
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil


Chemical and enzymatic catalytic agents act directly on the degradation of hydrogen peroxide (H2O2), increasing the aesthetic efficacy and reducing the cytotoxicity caused by the conventional in-office dental bleaching widely employed. Thus, the objective of the present study is to assess the influence of an experimental polymeric catalytic primer (PCP) containing 10 mg/mL of manganese oxide (MnO) applied to enamel on the aesthetic efficacy, degradation kinetics and trans-enamel and trans-dentin cytotoxicity of bleaching gels containing 10%, 20% and 35% H2O2. For this purpose, enamel and dentin discs will be stained and then bleached with both products. A gel with 35% H2O2 applied for 45 min on the enamel or no enamel treatment will be used as controls. To determine the aesthetic efficacy, the discs will be stained and the enamel covered with the PCP, on which the bleaching gels will be applied (UV reflection spectrophotometer, CIE L *a*b* system). After this step, the rate of H2O2 degradation will be evaluated, as well as the production of hydroxyl radicals (OH*). To assess the trans-enamel and trans-dentin cytotoxicity, the discs will be adapted to artificial pulp chambers and the bleaching protocols will be performed. The extracts (culture medium + bleaching gel components diffused through the enamel/dentin discs) will be collected and applied on cultured odontoblast-like MDPC-23 cells, which will be evaluated concerning their viability (MTT) and morphology (MEV). The quantification of the total H2O2 capable of diffusing across the discs will be also determined (leuco-crystal violet/peroxidase). The numerical data obtained employing all laboratory protocols will be subjected to specific statistical analysis. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; ZUTA, UXUA ORTECHO; MENDES SOARES, IGOR PAULINO; ANSELMI, CAROLINE; SOARES, DIANA GABRIELA; FRAGA BRISO, ANDRE LUIZ; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Manganese oxide increases bleaching efficacy and reduces the cytotoxicity of a 10% hydrogen peroxide bleaching gel. CLINICAL ORAL INVESTIGATIONS, v. 26, n. 12, p. 10-pg., . (20/08882-6, 21/01184-4)
DIAS, MARLON FERREIRA; MARTINS, BEATRIZ VOSS; DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; LEITE, MARIA LUISA; ORTECHO-ZUTA, UXUA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. A new approach for professional dental bleaching using a polymeric catalyst primer. Journal of Esthetic and Restorative Dentistry, v. N/A, p. 10-pg., . (20/08882-6)
MARTINS, BEATRIZ VOSS; DIAS, MARLON FERREIRA; DE OLIVEIRA RIBEIRO, RAFAEL ANTONIO; DE ALENCAR E SILVA LEITE, MARIA LUISA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. Innovative strategy for in-office tooth bleaching using violet LED and biopolymers as H2O2 catalysts. Photodiagnosis and Photodynamic Therapy, v. 38, p. 8-pg., . (20/08882-6)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
DIAS, Marlon Ferreira. Influência de um primer catalisador sobre a eficácia estética, cinética de degradação e toxicidade de géis clareadores. 2022. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araraquara Araraquara.

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