Advanced search
Start date
Betweenand

Potential of photodynamic therapy in modulating the inflammatory response and microbial virulence factors: evaluation in a model of three-dimensional co-cultures of gingival fibroblasts, Candida albicans and Staphylococcus aureus

Grant number: 20/07110-0
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2021
Effective date (End): February 29, 2024
Field of knowledge:Health Sciences - Dentistry - Dental Clinics
Principal Investigator:Fernanda Lourenção Brighenti
Grantee:Analú Barros de Oliveira
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil

Abstract

Increased resistance to antibiotics is one of the most urgent challenges for Medicine worldwide and requires the search for innovative and more efficient medical strategies. The treatment of ulcerated oral lesions represents a great challenge, because in addition to the important symptoms, oral ulcers are susceptible to colonization by microorganisms, and these lesions may be more painful, more extensive, and may have a prolonged duration or worsening of the condition clinical when the oral microflora is unbalanced and harmful bacteria are in increasing proliferation, which can penetrate the tissue and cause the emergence of systemic infectious conditions. Photodynamic therapy (PDT) presents itself as a promising alternative for the treatment of inflammatory and infectious skin processes. However, few studies have evaluated the effectiveness of PDT in ulcerated lesions of the oral cavity, and no study has evaluated its action on microorganisms related to these oral lesions. Thus, the present study is divided into three studies and aims to: 1) carry out a systematic review of the literature followed by a meta-analysis in order to assess the effectiveness of PDT under tissue disinfection of ulcerated lesions; 2) to evaluate the dose-response relationship of the concentration of photosensitizing agents (Photodithazine® and Senna reticulata plant extract), and of the light dose, using cell cultures in gingival fibroblast monolayer and 3) to evaluate the in vitro potential of mediated PDT by these photosensitizing agents to be used as a new therapeutic modality for the treatment and prevention of infectious processes present in oral ulcerated lesions. For this, a three-dimensional cell model of coc-culture of gingival fibroblasts, Candida albicans and Staphylococcus aureus will be made and the evaluation of microbial virulence factors and tissue inflammatory modulation will be performed using the qRT-PCR technique. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
More itemsLess items
Articles published in other media outlets ( ):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Scientific publications (12)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GABBAI-ARMELIN, PAULO R.; SALES, LUCIANA S.; FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; DE OLIVEIRA, JULIANA R.; GIRO, ELISA M. A.; BRIGHENTI, FERNANDA L.. A systematic review and meta-analysis of the effect of thymol as an anti-inflammatory and wound healing agent A review of thymol effect on inflammation and wound healing. Phytotherapy Research, v. 36, n. 9, p. 29-pg., . (20/07110-0, 19/08375-0, 21/01191-0, 19/26066-4)
DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; BASSO, FERNANDA GONCALVES; FONTANA, CARLA RAQUEL; APARECIDA GIRO, ELISA MARIA; BRIGHENTI, FERNANDA LOURENCAO. A systematic review and meta-analysis of the effect of photodynamic therapy for the treatment of oral mucositis. Photodiagnosis and Photodynamic Therapy, v. 34, . (18/18440-0, 20/07110-0, 21/01191-0, 19/08375-0, 18/23015-7)
DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FONTANA, CARLA RAQUEL; BASSO, FERNANDA GONCALVES; BRIGHETI, FERNANDA LOURENCAO. Photodynamic therapy for treating infected skin wounds: A systematic review and meta-analysis from randomized clinical trials. Photodiagnosis and Photodynamic Therapy, v. 40, p. 11-pg., . (13/07276-1, 18/23015-7, 21/01191-0, 20/07110-0, 19/08375-0, 18/18440-0)
FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; PALACON, MARIANA PARAVANI; SILVA, EVANIO VILELA; SGAVIOLI MASSUCATO, ELAINE MARIA; DE ALMEIDA, LUCIANA YAMAMOTO; LEON, JORGE ESQUICHE; BUFALINO, ANDREIA. The role of CD68+and CD163+macrophages in immunopathogenesis of oral lichen planus and oral lichenoid lesions. Immunobiology, v. 226, n. 3, . (20/07110-0, 16/11419-0, 17/01438-0, 18/18440-0)
GABBAI-ARMELIN, PAULO ROBERTO; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; SALES, LUCIANA SOLERA; BARBOSA, ELIS RODRIGUES OLIVEIRA; MIRANDA, MARINA LINS; SALOMAO, KARINA BORGES; BRIGHENTI, FERNANDA LOURENCAO. COVID-19 (SARS-CoV-2) infection and thrombotic conditions: A systematic review and meta-analysis. EUROPEAN JOURNAL OF CLINICAL INVESTIGATION, v. 51, n. 6, . (21/01191-0, 19/08375-0, 19/26066-4, 18/18440-0, 20/07110-0)
SALES, LUCIANA SOLERA; MIRANDA, MARINA LINS; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FONTANA, CARLA RAQUEL; MILWARD, MICHAEL; BRIGHENTI, FERNANDA LOURENCAO. Effect of the technique of photodynamic therapy against the main microorganisms responsible for periodontitis: A systematic review of in-vitro studies. ARCHIVES OF ORAL BIOLOGY, v. 138, p. 13-pg., . (19/26066-4, 21/12931-5, 20/07110-0, 19/08375-0, 21/01191-0)
FERRISSE, TULIO MORANDIN; DIAS, LUANA MENDONCA; DE OLIVEIRA, ANALU BARROS; JORDAO, CLAUDIA CAROLINA; DE OLIVEIRA MIMA, EWERTON GARCIA; PAVARINA, ANA CLAUDIA. Efficacy of Antimicrobial Photodynamic Therapy Mediated by Photosensitizers Conjugated with Inorganic Nanoparticles: Systematic Review and Meta-Analysis. PHARMACEUTICS, v. 14, n. 10, p. 18-pg., . (19/27634-6, 20/07110-0, 20/16227-8, 21/01191-0, 13/07276-1)
FORNEL, DIOGENES GERMANO; FERRISSE, TULIO MORANDIN; DE OLIVEIRA, ANALU BARROS; FONTANA, CARLA RAQUEL. Photodynamic Therapy Can Modulate the Nasopharyngeal Carcinoma Microenvironment Infected with the Epstein-Barr Virus: A Systematic Review and Meta-Analysis. BIOMEDICINES, v. 11, n. 5, p. 14-pg., . (22/12828-2, 21/01191-0, 13/07276-1, 20/07110-0)
SILVESTRE, AMANDA LETICIA POLLI; DOS SANTOS, ALINE MARTINS; DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; BRIGHENTI, FERNANDA LOURENCAO; MENEGUIN, ANDREIA BAGLIOTTI; CHORILLI, MARLUS. Evaluation of photodynamic therapy on nanoparticles and films loaded-nanoparticles based on chitosan/alginate for curcumin delivery in oral biofilms. International Journal of Biological Macromolecules, v. 240, p. 13-pg., . (21/01191-0, 18/23096-7, 20/07110-0)
DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; FRANCA, GABRIELA GOMES; DE ANNUNZIO, SARAH RAQUEL; KOPP, WILLIAN; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. Potential Use of Brazilian Green Propolis Extracts as New Photosensitizers for Antimicrobial Photodynamic Therapy against Cariogenic Microorganisms. PATHOGENS, v. 12, n. 2, p. 19-pg., . (19/08375-0, 18/23015-7, 18/18440-0, 13/07276-1, 21/01191-0, 20/07110-0)
DE OLIVEIRA, ANALU BARROS; FERRISSE, TULIO MORANDIN; DE ANNUNZIO, SARAH RAQUEL; FRANCA, MARIA GLEIZIANE ARAUJO; SILVA, MARIA GORETTI DE VASCONCELOS; CAVALHEIRO, ALBERTO JOSE; FONTANA, CARLA RAQUEL; BRIGHENTI, FERNANDA LOURENCAO. In Vitro Evaluation of Photodynamic Activity of Plant Extracts from Senna Species against Microorganisms of Medical and Dental Interest. PHARMACEUTICS, v. 15, n. 1, p. 16-pg., . (13/07600-3, 22/12828-2, 14/50926-0, 20/07110-0, 19/08375-0, 18/09088-1, 18/18440-0, 18/23015-7, 21/01191-0)
FERRISSE, TULIO MORANDIN; DIAS, LUANA MENDONCA; DE OLIVEIRA, ANALU BARROS; JORDAO, CLAUDIA CAROLINA; DE OLIVEIRA MIMA, EWERTON GARCIA; PAVARINA, ANA CLAUDIA. Efficacy of curcumin-mediated antibacterial photodynamic therapy for oral antisepsis: A systematic review and network meta-analysis of randomized clinical trials. Photodiagnosis and Photodynamic Therapy, v. 39, p. 9-pg., . (20/07110-0, 21/01191-0, 13/07276-1, 20/16227-8)

Please report errors in scientific publications list using this form.