Scholarship 20/16000-3 - Bioluminescência, Fungos - BV FAPESP
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Functional characterization of candidate genes for the biological clock of Neonothopanus gardneri and its relationship with bioluminescence

Grant number: 20/16000-3
Support Opportunities:Scholarships in Brazil - Doctorate (Direct)
Start date until: January 01, 2021
End date until: September 30, 2024
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal Investigator:Cassius Vinicius Stevani
Grantee:Bianca de Barros Nóbrega
Host Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:17/22501-2 - Electronic chemiexcitation in biological systems: bioluminescence and photochemistry in the dark, AP.TEM

Abstract

Basidiomycete bioluminescence and the ascomycete biological clock system have been described in the literature. With this project, we want to understand the regulation of basidiomycete bioluminescence and develop biotechnological applications. Initially, a N. gardneri genetic transformation protocol will be implemented and later on, the cultivation of mutant N. gardneri strains for clock candidate genes. Through molecular biology techniques such as RT-qPCR and CRISPR-Cas9 technology, we aim to discover which are the candidates for clock genes of this species, verifying their relationship with the rhythmicity and bioluminescence in fungi. (AU)

News published in Agência FAPESP Newsletter about the scholarship:
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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SOARES, DOUGLAS M. M.; PROCOPIO, DIELLE P.; ZAMUNER, CAIO K.; NOBREGA, BIANCA B.; BETTIM, MONALISA R.; DE REZENDE, GUSTAVO; LOPES, PEDRO M.; PEREIRA, ARTHUR B. D.; BECHARA, ETELVINO J. H.; OLIVEIRA, ANDERSON G.; et al. Fungal bioassays for environmental monitoring. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, v. 10, p. 6-pg., . (19/12605-0, 17/22501-2, 20/16000-3, 19/21782-3)
NOBREGA, BIANCA B.; SOARES, DOUGLAS M. M.; ZAMUNER, CAIO K.; STEVANI, V, CASSIUS. Optimized methodology for obtention of high-yield and-quality RNA from the mycelium of the bioluminescent fungus Neonothopanus gardneri. Journal of Microbiological Methods, v. 191, . (20/16000-3, 19/12605-0, 19/21782-3, 17/22501-2)

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