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Functional characterization of candidate genes for the biological clock of Neonothopanus gardneri and its relationship with bioluminescence

Grant number: 20/16000-3
Support type:Scholarships in Brazil - Doctorate (Direct)
Effective date (Start): January 01, 2021
Effective date (End): August 31, 2023
Field of knowledge:Biological Sciences - Biophysics - Radiology and Photobiology
Principal researcher:Cassius Vinicius Stevani
Grantee:Bianca de Barros Nóbrega
Home Institution: Instituto de Química (IQ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:17/22501-2 - Electronic chemiexcitation in biological systems: bioluminescence and photochemistry in the dark, AP.TEM

Abstract

Basidiomycete bioluminescence and the ascomycete biological clock system have been described in the literature. With this project, we want to understand the regulation of basidiomycete bioluminescence and develop biotechnological applications. Initially, a N. gardneri genetic transformation protocol will be implemented and later on, the cultivation of mutant N. gardneri strains for clock candidate genes. Through molecular biology techniques such as RT-qPCR and CRISPR-Cas9 technology, we aim to discover which are the candidates for clock genes of this species, verifying their relationship with the rhythmicity and bioluminescence in fungi. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
NOBREGA, BIANCA B.; SOARES, DOUGLAS M. M.; ZAMUNER, CAIO K.; STEVANI, V, CASSIUS. Optimized methodology for obtention of high-yield and-quality RNA from the mycelium of the bioluminescent fungus Neonothopanus gardneri. Journal of Microbiological Methods, v. 191, DEC 2021. Web of Science Citations: 0.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.