Advanced search
Start date
Betweenand

Analysis of the double heterozygous Hspg2+/- Fbn1+/mg”loxPneo murine model for skeletal and vascular phenotypes variability studies in Marfan Syndrome

Grant number: 20/15779-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2021
Effective date (End): April 30, 2022
Field of knowledge:Biological Sciences - Genetics - Human and Medical Genetics
Principal Investigator:Lygia da Veiga Pereira
Grantee:Glauco de Oliveira Gavioli Ferreira
Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil

Abstract

The Marfan syndrome (MFS) is a hereditary and pleiotropic genetic condition characterized by connective tissue anomalies, of which clinical manifestations occur mainly in the skeletal, cardiovascular and ocular systems. Due to mutations in the FBN1 gene that encodes the fibrilin-1 extracellular matrix glycoprotein, the MFS presents variable expressiveness that results in an large clinical variability even within the same family and without clear genotype-phenotype correlations, which suggests the existence of marfanoid phenotype modifying genes. Linkage experiments conducted in the mg”loxPneo murine model for MFS revealed loci associated with the severity of the skeletal and cardiovascular phenotypes in which the contiguity of two of them, one related to the skeletal (Awtq1) and the other to the cardiovascular phenotype (Krq2), suggests the influence of a common gene in both phenotypes. Hence, the Hspg2 gene that encodes perlecan a heparan sulfate proteoglycan that interacts directly with fibrillin-1 was identified at the Awtq1 locus. Positive correlations were also found between the expressions of Fbn1 and Hspg2 in mg”loxPneo mice from different backgrounds, suggesting the cooperation of these two genes, just as less expression of Hspg2 was observed in those animals with more severe skeletal and cardiovascular phenotypes. Finally, a double heterozygous Hspg2+/- Fbn1+/mg”loxPneo murine model was developed for future studies. Therefore, this project hypothesizes that the low expression of the HSPG2 gene leads to more severe skeletal and vascular phenotypes in MFS, which will be tested by comparing the intensity of these phenotypes between the mg”loxPneo and the Hspg2+/- Fbn1+/mg”loxPneo models. Quantitative analyzes of both phenotypes and the correlation between immunofluorescences and echocardiography will be performed in both groups.

News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
More itemsLess items
VEICULO: TITULO (DATA)
VEICULO: TITULO (DATA)

Please report errors in scientific publications list using this form.