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Characterization of the local and systemic immune response of immunized cattle with a multi-antigenic anti-R. microplus vaccine for the determination of immunological markers of tick protection

Grant number: 20/04990-9
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): January 01, 2021
Effective date (End): July 31, 2023
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Preventive Veterinary Medicine
Principal researcher:Beatriz Rossetti Ferreira
Grantee:Natalia Serra Mendes
Home Institution: Escola de Enfermagem de Ribeirão Preto (EERP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated research grant:15/09683-9 - Development of a recombinant multicomponent chimeric vaccine based in protein epitopes from Rhipicephalus microplus ticks, AP.TEM


Rhipicephalus microplus ticks are considered one of the most harmful cattle parasites, responsible for significant losses in the livestock sector, especially in tropical and subtropical countries. Problems related to the use of chemical acaricides point to the need for sustainable alternatives for the control of bovine ticks. Our research group performed experimental immunizations with a combination of nine recombinant antigens derived from the saliva of R. microplus, which demonstrated potential vaccine in cattle, since it generated a protective response of 78% effectiveness. Complementary studies may indicate which cell populations and immune system mediators are being modulated by immunization and by which mechanisms this modulation occurs. Thus, this project proposes, for the first time, to clarify the cutaneous and systemic immunological mechanisms involved in the protective immunity conferred by an anti-R. microplus vaccine formulation applied in bovines. To this end, we will perform a histopathological characterization of the skin by means of histological and immunohistochemical assays, quantification of antibodies and cytokines in the blood, evaluation of the transcriptional profile of immunological cells in the blood and skin of immunized and tick infested cattle, from flow cytometry (FACS) followed by sequencing of RNA (FACS-RNAseq) and analysis in the context of systems vaccinology. The results may assist in elucidating the mechanisms of vaccine protection, enabling the identification of immunological correlates of protection that can endorse the rational design of more efficient and safer tick vaccines. (AU)

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