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Gene expression during early pregnancy in Bubalus bubalis

Grant number: 20/10732-2
Support type:Scholarships abroad - Research Internship - Doctorate
Effective date (Start): February 01, 2021
Effective date (End): August 31, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:Eunice Oba
Grantee:Viviane Maria Codognoto
Supervisor abroad: Ronaldo Luis Aoki Cerri
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade Estadual Paulista (UNESP). Campus de Botucatu. Botucatu , SP, Brazil
Research place: University of British Columbia (UBC), Canada  
Associated to the scholarship:18/13338-3 - Serum and embryonic protein of gestant buffaloes, BP.DR

Abstract

The pursuance of better reproductive outcomes has driven for the selection of animals that are genetically superior through the utilization of varying reproductive techniques. However, in buffalo, several different factors impact their reproductive performance, such as the long interval between parturitions observed in the species, and failures in the detection of estrus due to low expression of estrual behaviors. For an improvement in reproductive outcomes, it is necessary to comprehend the biologic and genetic mechanisms that are related to early pregnancy, contributing to the understanding of different phenomena surrounding different reproductive phases , and use this knowledge to help move towards improvements in fertility. Early diagnosis of pregnancy is a practice that can increase the reproductive efficiency of farms by reducing the interval between inseminations and thus parturitions. However, methods already used in cattle, such as rectal palpation, ultrasonography and quantifying concentrations of progesterone or pregnancy-associated glycoproteins, are often methods that cannot be identified in early pregnancy in buffalo, and also demand proper equipment and knowledge to be able to obtain results with high accuracy and precision. Although, several studies have been developed with the aim to identify a reliable and low cost method to diagnose pregnancy using biomarkers available in bodily fluids with easy access. Therefore, the objective of our study is to describe and correlate genes expressed in blood, the corpus luteum, caruncle and endometrium of buffalo which can be used for early pregnancy diagnosis in the species. (AU)

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