Structural analysis and relative expression of the HCMV IL0 isoforms in glioblastoma samples

Abstract

Human Cytomegalovirus (HCMV) belongs to the Herpesviridae family,Betaherpesvirinae subfamily, which includes neurotropic viruses, with long replicative cycle and restricted species specificity. HCMV is an agent with high worldwide prevalence endowed with an exceptionally large arsenal of coded proteins which evade the host's innate and adaptive immune defenses, promoting viral persistence and latency throughout the host's life. Latency is characterized by the presence of viral DNA specific cell types with absence of viral replication and particle formation. A group of immunomodulatory proteins expressed by HCMV are homologous of host cytokines and chemokines. The cellular interleukin-10 homologue (cIL-10), called viral IL-10 (cmvIL-10). The coding gene of cmvIL-10, UL111A, undergoes alternative splicing producing different isoforms. The transcript cmvIL10 (Isoform A) is expressed mainly during the lytic cycle, while LAcmvIL10 (Isoform B) is expressed in the lytic cycle and during latency. In addition, to these two transcripts there are another 5 transcripts (C - G) reported. However, little information about its functions and structures are known. The present project aims, the computational modeling and interaction analysis between the isoforms A and B with the il10 cell receptor. In addition to the comparation of IL-10 expression of isoforms A, B, E and F.