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Biosynthesis of roussoellatide, a polyketide isolated from the fungus Roussoella sp. DLM-33

Grant number: 20/11691-8
Support type:Scholarships abroad - Research Internship - Doctorate (Direct)
Effective date (Start): December 01, 2020
Effective date (End): November 30, 2021
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Organic Chemistry
Principal researcher:Roberto Gomes de Souza Berlinck
Grantee:Darlon Irineu Bernardi
Supervisor abroad: Russell John Cox
Home Institution: Instituto de Química de São Carlos (IQSC). Universidade de São Paulo (USP). São Carlos , SP, Brazil
Research place: Leibniz Universität Hannover, Germany  
Associated to the scholarship:16/21341-9 - Hydrophilic and bioactive secondary metabolites produced by filamentous fungi, BP.DD

Abstract

Investigation of Roussoella sp. DLM-33 cultures revealed a new polyketide, roussoellatide,which encompasses an unprecedented carbon skeleton. The biosynthetic pathway proposed for roussoellatide was investigated by feeding experiments with labeled precursors. The hypothetical pathway requires at least two Favorskii-like rearrangements and an intermolecular [4+2]cycloaddition. Such reactions lack biochemical characterization. The principal aim of this proposal is to shed light on the genes encoding Favorskii oxidases and Diels-Alderases using genome mining and genetic engineering. Knock-out mutants will be generated to confirm the association of candidate genes with roussoellatide biosynthesis. A deep investigation of the unique roussoellatide biosynthesis will expand the scope of uncommon biosynthesis enzymes of fungal secondary metabolism. (AU)

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