Influence of Salmonella enteritidis and Salmonella typhimurium under gut microbiome from broilers, after deletion of pduA and ttrA genes: a genomic approach

Abstract

It is common the emergence of human foodborne diseases outbreaks worldwide caused by the consumption of poultry products contaminated with Salmonella Enteritidis (SE) and Salmonella Typhimurium (ST). Experiments performed with mammals has shown that enteric salmonellas are able to use the inflammatory process provoked by them as a source of energy to survive and multiply inside the gut. This process is associated with the metabolism of tetrathionate (ttr) as a by-product of the host inflammatory gut response. After ttr depletion, the pathogen makes use of propanediol (pdu) as an energy source. Therefore, the intestinal inflammatory response of the host promotes efficient bacterial multiplication in the intestinal lumen, with consequent colonization and increased fecal-oral transmission. In view of the importance of poultry foods in foodborne salmonellosis we will investigate the relevance of genes related to tetrathionate and propanediol metabolism to the intestinal colonization of Salmonella in chickens. In our previous study, we proposed to construct SE, ST and SH strains containing the deletions of ttrA and pduA genes to assess their behaviour during the infection. However, preliminary results showed that the SE mutant were excreted among feces and provoked systemic infection longer than wild-type strain. To clarify the obtained data, we propose to carry out a broiler infection with both STM and SE mutants followed by metagenomic and metabolomic approaches. The knowledge acquired may clarify the relationship between the mutant bacteria and the gut microbiome of the host. (AU)