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Molecular characterization of Mazama rondoni (Miranda Ribeiro, 1914)

Grant number: 20/02744-0
Support type:Scholarships abroad - Research Internship - Master's degree
Effective date (Start): October 30, 2020
Effective date (End): February 01, 2021
Field of knowledge:Biological Sciences - Genetics - Animal Genetics
Principal researcher:José Maurício Barbanti Duarte
Grantee:Gabrielle Queiroz Vacari
Supervisor abroad: Susana Gonzalez
Home Institution: Faculdade de Ciências Agrárias e Veterinárias (FCAV). Universidade Estadual Paulista (UNESP). Campus de Jaboticabal. Jaboticabal , SP, Brazil
Research place: Instituto de Investigaciones Biológicas Clemente Estable (IIBCE), Uruguay  
Associated to the scholarship:19/05420-4 - Morphological, cytogenetic and molecular characterization of Mazama rondoni (Miranda Ribeiro, 1914), BP.MS

Abstract

Mazama rondoni is considered synonymous with Mazama nemorivaga due to the absence of significant morphological differences, however, the morphological characteristics of the genus Mazama are very similar, making it necessary to use genetic analyzes. Museum specimens are widely used to solve taxonomic problems through morphological and molecular studies, in which museum collections DNA has been used to validate species, through the combination of ancient holotype DNA analysis and life animal data free. Within this context, the present work aims to identify through ancient DNA from museum samples to describe Mazama rondoni, through molecular analyzes. Samples of turbinal bones and skins were obtained from the National Museum of Rio de Janeiro and the Museum of Zoology at USP for DNA extraction. Five fragments of mitochondrial DNA will be amplified (Cit-b 224pb, Cit-b 305pb, D-loop 305pb, ND5 249pb, ND5 249pb) and the data obtained will finally be analyzed phylogenetically, thus obtaining the identification of the Mazama rondoni species for its revalidation.

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