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Gene expression from samples FFPE of skin wound of horses treated with copaiba oil

Grant number: 20/06571-3
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2020
Effective date (End): August 31, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Clinics and Surgery
Principal researcher:Flávia de Almeida Lucas
Grantee:Mariana Zacarin Guiati
Home Institution: Faculdade de Medicina Veterinária (FMVA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil

Abstract

he aim of this study was to evaluate type I and III collagen gene expression from "FFPE" samples from experimental wounds in the lumbar and metacarpal regions of horse members treated with topical application of Copaíba Oil. Biopsies of the skin wounds performed on four horses will be used on days three and 21 after their surgical preparation. These samples were fixed in formalin and embedded in paraffin (FFPE) in a previous project. With the use of specific kits for RNA extraction from paraffin samples, RNA will be extracted from each sample for real-time Quantitative PCR analysis (RT qPCR). The data will be subjected to analysis of variance with repeated measures between the studied moments, followed by the F test.

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