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Investigation of the effect of chronic fluoride intake on insulin resistance with type 2 Diabetes induced by diet and streptozotocin

Grant number: 20/08138-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2020
Effective date (End): December 31, 2021
Field of knowledge:Health Sciences - Dentistry - Social and Preventive Dentistry
Principal Investigator:Heloisa Aparecida Barbosa da Silva Pereira
Grantee:Laura Ribeiro
Host Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil


Diabetes mellitus is considered to be a disease of great impact and prospects are likewise expected to increase a lot in the coming years, due to people's lifestyle. Among the two types of diabetes, type 2 (DM2) is the one with the highest prevalence (90%). Some resistant studies that fluoride (F) can induce insulin resistance / sensitivity. Thus, the aim of this study is to investigate the effects of chronic F intake in relation to insulin resistance in diabetic mice induced by a high-fat diet and streptozotocin. For this purpose, 30 adult male mice of the C57BL / 6J strain were used, which were divided into 3 experimental groups (n = 10), according to the dose of F (0.10 or 50 ppm) administered through drinking water. The animals received a high-fat diet for 8 weeks or more and after reaching a weight equal to or greater than 30g, they received daily intraperitoneal injections of streptozotocin (STZ) for 3 to 5 days. From the following week, after DM2 (glycemia greater than 200mg / dl) was found, treatment with F began, lasting 21 days. Blood glucose tests were performed periodically to check for glycemic changes, in order to detect insulin resistance, after the administration of STZ and confirmation of DM2. After the experimental period, the animals were euthanized, and blood was collected to measure F, glucose and insulin. How they will be, what will be the focus of the present work, have already been collected and stored. For a fluorine analysis in plasma, the pre-diffusion technique by Taves (1996) will be used and analyzed with the specific electrode Orion 9409. The glucose analysis without plasma will be performed by the glucose-oxidase method and the insulin with a ELISA. After checking the normality and homoscedasticity of the data, the same criteria found for the appropriate analysis (p <0.05).

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