Development and application of calcium silicate nanoparticles for dental remineralization

Abstract

Application of bioactive glasses, calcium silicates and calcium orthophosphates particles on dental adhesives are an effective strategy to reduce collagen enzymatic degradation at the hybrid layer and to promote apatite precipitation at dentin-adhesive interface. Therefore, this project aims to: 1) investigate the effect of sol-gel synthesis variables over Calcium Silicate (CS) nanoparticles characteristics. Nanoparticles will be characterized by the obtained phase (XRD), density (helium pycnometry), surface area (BET), size distribution (laser scattering) and morphology (scanning electron microscopy and transmission electron microscopy); 2) evaluate the effect of particles incorporation to a commercial dental adhesive on the degree of conversion and ions release; 3) evaluate, after 24 hours and 12 months of storage in Simulated Body Fluid (SBF), the effect of adding CS to the comercial adhesive system or to an experimental primer in relation to nanohardness/elastic modulus of the adhesive interface, collagenolytic activity of metalloproteinases (in situ zymography assay) and scanning electron microscopy/energy dispersive x-ray spectroscopy (SEM/EDS) of the interface. Data will be subjected to ANOVA (Analysis of Variance). In all cases, Tukey test will be used for pair-wise comparisons. The global significance level (±) will be set at 5%. (AU)