The traditional techniques for the preparation of the samples for toxicological analyses usually involve several steps. Therefore, those spend a lot of time and resources, as the volume of the samples as the organic solvents. To solve this issue, new microextraction techniques were developed, among them the dispersive liquid-liquid microextraction (DLLME), which besides allowing to use of minimal volumes of the sample and the solvents, can also be realized in fewer steps than the traditional methods, making the extraction and analysis much easier. The present study has as its objective to develop and validate an analytical method, based on DLLME technique and gas chromatography-mass spectrometry (GC-MS/MS), for determination of ketamine, norketamine, and 6-hydroxy-norketamine in oral fluid samples. The developed method can be used to perform laboratory analysis of intoxications caused by those toxic agents, and for field researches on parties, about the use of ketamine as an abused drug.
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