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Development of an analytical method for the determination of ketamine and its metabolites in oral fluids samples using liquid-liquid dispersive microextration (DLLME)

Grant number: 20/07470-6
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): September 01, 2020
Effective date (End): August 31, 2021
Field of knowledge:Health Sciences - Pharmacy - Toxicological Analysis
Principal Investigator:José Luiz da Costa
Grantee:Júlia Martinelli Magalhães Kahl
Host Institution: Faculdade de Ciências Farmacêuticas (FCF). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:17/02147-0 - Single drop chromatography and its coupling to mass spectrometry: instrumental strategies, development of materials, automation and analytical applications, AP.TEM


The traditional techniques for the preparation of the samples for toxicological analyses usually involve several steps. Therefore, those spend a lot of time and resources, as the volume of the samples as the organic solvents. To solve this issue, new microextraction techniques were developed, among them the dispersive liquid-liquid microextraction (DLLME), which besides allowing to use of minimal volumes of the sample and the solvents, can also be realized in fewer steps than the traditional methods, making the extraction and analysis much easier. The present study has as its objective to develop and validate an analytical method, based on DLLME technique and gas chromatography-mass spectrometry (GC-MS/MS), for determination of ketamine, norketamine, and 6-hydroxy-norketamine in oral fluid samples. The developed method can be used to perform laboratory analysis of intoxications caused by those toxic agents, and for field researches on parties, about the use of ketamine as an abused drug.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
DA CUNHA, KELLY FRANCISCO; MAGALHAES KAHL, JULIA MARTINELLI; FIORENTIN, TAIS REGINA; OLIVEIRA, KARINA DINIZ; COSTA, JOSE LUIZ. High-sensitivity method for the determination of LSD and 2-oxo-3-hydroxy-LSD in oral fluid by liquid chromatography-tandem mass spectrometry. Forensic Toxicology, v. 40, n. 2, p. 10-pg., . (20/07470-6, 18/11849-0, 20/10809-5, 18/00432-1)

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