Speciation of enterobacteria and other Gram-negative bacteria less frequent in clinical bovine mastitis cases diagnosed by mass spectrometry

Abstract

Speciation of enterobacteria and other gram-negative bacteria less frequent in clinical bovine mastitis infections in dairy cows diagnosed by mass spectrometry: Escherichia coli and Klebsiella pneumoniae are enterobacteria more frequently diagnosed in clinical mastitis cases of dairy cows, from the environmental origin. Nevertheless, other enterobacteria and gram-negative species are involved in mammary infections of cows and, it is possible that these pathogens are neglected, unnoticed or identified only in the level of genera, e.g., Proteus, Pseudomonas, Pasteurella, Enterobacter, Serratia, Hafnia, and Citrobacter. Besides commonly misdiagnosed, these groups of pathogens also induce high severity of clinical mastitis, shown multidrug resistance to conventional antimicrobials, and deserve public health issue, since may cause human diseases by transmission to human by milk and milk products. In this scenario, the aim of the present study is to investigate less frequent enterobacteria and other gram-negative bacterial species involved in clinical mammary infections of dairy cows, diagnosed based on mass spectrometry (matrixassisted laser desorption ionization timeofflight - MALDI-TOF). In addition, in vitro multidrug resistance of isolates to antimicrobials commonly used in the treatment of lactation and/or dry-off therapy of cows with mastitis will be investigated as well.