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Microbial proteases action on milk from animals with A1 and A2 alleles for ²-CN

Grant number: 20/01742-4
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): August 01, 2020
Effective date (End): July 31, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Products of Animal Origin Inspection
Principal researcher:Ana Maria Centola Vidal
Grantee:Arthur Firmino de Macedo Santos
Home Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil


Among milk proteins, caseins are the majority, in which ²-casein is the largest participant. This protein can be presented in several variants, with A1 and A2 being the most common in a dairy herd. The casein micelles are made of ± and ²-casein in its interior and º-casein on the outside, as a protection. Enzyme action upon º-casein is able to separate its two subunits, giving rise to the glycomacropeptide. In addition to enzymatic milk coagulation by renin, used in industries, milk most found microorganisms as Bacillus cereus and Pseudomonas fluorescents produce enzymes that have proteolytic action upon º-casein. This present project aims to verify the action of microbial proteases on milk from animals with different genotypes (A1A1, A1A2, A2A2) by experimental contamination of these milks with Bacillus cereus and Pseudomonas fluorescents and the quantification of GMP in different periods and temperatures of incubation (AU)

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