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Role of Saccharomyces boulardii in the development of cardiomyopathy in an experimental diabetes model: histomorphometric evaluation

Grant number: 19/20589-5
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): May 01, 2020
Effective date (End): April 30, 2021
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:Tatiana de Sousa da Cunha Uchiyama
Grantee:Sarah Gomes Ferreira
Host Institution: Instituto de Ciência e Tecnologia (ICT). Universidade Federal de São Paulo (UNIFESP). Campus São José dos Campos. São José dos Campos , SP, Brazil


Diabetic cardiomyopathy is a disease of the heart, diabetes mellitus (DM) and is not related to vascular, valvular or systemic arterial hypertension. The risk of triglycerides and cholesterol of the cardiac disease of collagen, the concentration of triglycerides and cholesterol, in which the deterrence of inflation and reduction of bioavailability of nitric oxide. Saccharomyces boulardii (S. boulardii) is a microorganism with probiotic effect capable of modulating the innate and adaptive system, with pro-inflammatory responses and activating as anti-inflammatory. The present study should evaluate the presence of cardiac hypertrophy, collagen deposition and degree of vascular complication in streptozotocin-induced diabetic (STZ) mice treated with Saccharomyces boulardii. 5-week-old male isogenic C57BL / 6 mice will be used from the Center for the Development of Experimental Models for Biology and Medicine (CEDEME). The animals are randomly divided into four experimental groups (n = 6 / group): A. Control group (C) - Animals receiving intraperitoneal injection of citrate buffer (placebo) and daily gavage for 8 consecutive weeks; B. Diabetic group (DM) - Animals receiving intraperitoneal injection of STZ and daily gavage for 8 consecutive weeks; C. Control group + Sb (CSb) - Animals receiving intraperitoneal injection of citrate buffer (placebo) and daily gavage with Sb for 8 consecutive weeks; D. STZ + Sb Group (DMSb) - Animals receiving intraperitoneal injection of STZ and daily Sb grooming for 8 consecutive weeks. At the end of the experimental protocol, the animals will be killed by decapitation and the cardiac tissue will be processed according to the routine to perform the proposed histological analyzes. Will be evaluated: body mass and blood glucose weekly; the mass of the heart and left ventricle at the end of the protocol; the cardiac hypertrophy index; the diameter of the cardiomyocytes; the intensity of collagen deposition in the left ventricle and the degree of vascular congestion in the left ventricle.

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