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Influence of the gaseous atmosphere on the expression of genes regulating epigenetic changes during in vitro cell reprogramming (cell generation iPSCs)

Grant number: 20/03487-1
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): April 01, 2020
Effective date (End): March 31, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Fabiana Fernandes Bressan
Grantee:Jessica Brunhara Cruz
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated research grant:15/26818-5 - Investigation of cellular and molecular mechanisms on in vitro induced toti- and pluripotency acquisition - a translational approach, AP.JP

Abstract

Analyze the efficiency and molecular characterization of cells reprogrammed in vitro (pluripotency-induced cells, or iPS cells) for pluripotency and epigenetics. Such cells will then be classified and used in the future for processes of cell differentiation, or for the production of bovine embryos. With this proposal, through the manipulation of the somatic cell culture environment, the change in epigenetic patterns that lead to the most effective and complete process of cell reprogramming is intended.

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