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Bisphosphonates and implantology: effect of metalloproteinases inhibitors in in vitro model

Grant number: 19/16886-4
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): March 01, 2020
Field of knowledge:Health Sciences - Dentistry - Periodontology
Principal Investigator:Fernanda Gonçalves Basso
Grantee:Laís Medeiros Cardoso
Host Institution: Faculdade de Odontologia (FOAr). Universidade Estadual Paulista (UNESP). Campus de Araraquara. Araraquara , SP, Brazil
Associated scholarship(s):22/03788-7 - Development of hydrogel scaffold containing the flavonoid naringenin to modulate peri-implant tissue repair: an in vitro study, BE.EP.DR


Bisphosphonates are indicated for treatment of osteolytic diseases, aiming to control bone resorption. These drugs show high affinity to mineral tissues and are released during traumatic or inflammatory events, leading to cytotoxic and cytopatic effects. For this reason, the installation of oral osseointegrated implants is still controversial for patients ongoing bisphosphonates treatment. Besides the cytotoxic effects, bisphosphonates may also induce the overexpression of matrix metalloproteinases (MMPs), negatively interfering on osseointegration and biological sealing. Therefore, the modulation of these enzymes could favor these tissue events. The aim of this study will be to evaluate the effect of two MMPs inhibitors - proanthocyanidins and naringenin, on osteoblasts and fibroblasts seeded onto titanium surfaces and treated with Zoledronic Acid (ZA). Titanium discs will be placed in cell-culture plates and cells will be seeded onto them. After 24 hours, ZA will be applied and after 24 or 48 hours cell proliferation, adhesion, collagen synthesis, alkaline phosphatase activity, gene expression, synthesis and activity of MMPs will be evaluated. Expression of TIMP-1 and -2 will also be assessed. Results will be statistically evaluated according to data distribution. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BASSO, FERNANDA GONCALVES; CARDOSO, LAIS MEDEIROS; RIBEIRO, ISABELA MASSARO; RIZZI, ELLEN; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO. nfluence of bisphosphonates on oral implantology: Sodium alendronate and zoledronic acid enhance the synthesis and activity of matrix metalloproteinases by gingival fibroblasts seeded on titaniu. ARCHIVES OF ORAL BIOLOGY, v. 127, . (18/11211-6, 19/16886-4)
CARDOSO, LAIS MEDEIROS; PANSANI, TAISA NOGUEIRA; HEBLING, JOSIMERI; DE SOUZA COSTA, CARLOS ALBERTO; BASSO, FERNANDA GONCALVES. hemotherapy drugs and inflammatory cytokines enhance matrix metalloproteinases expression by oral mucosa cell. ARCHIVES OF ORAL BIOLOGY, v. 127, . (18/11211-6, 19/16886-4)

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