Influence of MCT1 and MCT4 polymorphism on blood responses associated to performance against a 1 km time trial cycling test in recreational athletes

Abstract

High intensity exercise increases muscle energy demand, causing adenosine-5-triphosphate (ATP) to be hydrolyzed to adenosine-5-diphosphate (ADP), leading to a decompensation between glycolytic rate and pyruvate oxidation, resulting in production of lactic acid to facilitate the continuation of muscle contraction. Lactic acid will be dissociated into lactate anion (Lac-) and hydrogen cation (H +). The accumulation of hydrogen ions causes acidification of the environment, which contributes to muscle fatigue and consequent drop in physical performance. MCT1 and MCT4 transporters are responsible for the efflux of Lac- and H + out of myocytes. It is suggested that exercise increases the expression and activity of these transporters, however, few studies have demonstrated the influence of polymorphisms on the genes that encode these transporters in the performance against a high intensity exercise. Therefore, the study will analyze the influence of polymorphism on these genes on physical performance in a 1-km time trial. The study in question will recruit 42 physically active, healthy, ergogenic adult male volunteers. A 24-hour food recall will be held. In addition to determining the genotype via real-time PCR, blood gas analysis and metabolite analysis will be performed during an incremental test and 1-km time trial. Volunteers will attend the lab in four moments: 1) Incremental testing and familiarization of the 1-km time trial. 2) Familiarization 2 of the 1-km time trial and genotyping blood will still be collected; 3 and 4) Main tests where there will be the 1-km time trial and blood collection for metabolite analysis. Data analysis will be conducted using SAS software version 9.3. The assumed significance level will be p <0.05. The results of this study will provide new data on the influence of these transporter polymorphisms on sports performance, providing important information for future research and prescribing individualized training.