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Association between tumor necrosis factor-alpha polymorphisms and their receptors and post-treatment apical periodontitis persistence

Grant number: 19/16038-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2020
Effective date (End): January 31, 2022
Field of knowledge:Health Sciences - Dentistry - Endodontics
Principal Investigator:Manoel Damiao de Sousa Neto
Grantee:Guilherme Piedade Assed de Castro
Host Institution: Faculdade de Odontologia de Ribeirão Preto (FORP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Genetic polymorphisms are differences in the sequence of human DNA that can alter gene expression and influence the organism's susceptibility to disease as well as its responses to the environment. Thus, in addition to microbial and mechanical factors related to the root canal itself, factors intrinsic to the host may interfere with the success of endodontic treatment and the need for reintervention. This study will investigate molecular aspects involved in the persistence of post-treatment periapical lesions by collecting saliva samples to compose a DNA bank, extracting genomic material, clinical and radiographic evaluation, and genotyping by PCR allelic discrimination analysis in real-time. Clinical and radiographic examinations will be used as phenotypic parameters to understand the molecular aspects involved in the etiology of persistent endodontic infection. Patients diagnosed with pulpal necrosis who still present persistent periapical lesion (n = 200) and patients with repair of the lesion (control group n = 200) will be included in the study 12 months after endodontic treatment. Saliva samples recently collected, as well as those that are already stored in the bank will be used as a source of genomic DNA. After sample processing, genotyping for the soluble tumor necrosis factor-alpha (TNF) and TNF-± receptor 1 and 2 (TNFRSF1A and TNFRSF1B, respectively) mediators will be performed by real-time PCR. Chi-square, Fisher's exact and Odds Ratio calculations will be used to assess whether any genotype or allele is associated with the persistent periapical lesion. The significance level established will be 5%.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
PIEDADE DE CASTRO, GUILHERME ASSED; FERREIRA PETEAN, IGOR BASSI; GARCIA DE PAULA-SILVA, FRANCISCO WANDERLEY; KUCHLER, ERIKA CALVANO; ANTUNES, LEONARDO DOS SANTOS; BEZERRA SEGATO, RAQUEL ASSED; BEZERRA DA SILVA, LEA ASSED; CORREA, SILVA-SOUSA ALICE; SOUSA-NETO, MANOEL DAMIAO. Genetic polymorphism in the tumour necrosis factor alpha gene (G-308A) is associated with persistent apical periodontitis in Brazilians. International Endodontic Journal, v. 56, n. 1, p. 10-pg., . (19/16038-3)

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