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Use of beta-tricalcium phosphate in conjunction with natural latex membrane on guided bone regeneration of bone defected in tibia of osteopenic rats

Grant number: 19/11958-7
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): February 01, 2020
Effective date (End): September 30, 2021
Field of knowledge:Health Sciences - Physiotherapy and Occupational Therapy
Principal Investigator:Antônio Carlos Shimano
Grantee:Guilherme Jafroni Alves Silva
Host Institution: Faculdade de Medicina de Ribeirão Preto (FMRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil


Bone healing is a complex process involving specific steps. When these are not well performed, they may compromise the bone composition. One of the factors that cause osteometabolic changes in bone healing is osteoporosis. Due to the problems of regeneration of the bone tissue, several resources have been developed to improve the tissue recomposition of the bone. The natural latex membrane, synthesized from the natural latex extracted from the rubber tree Hevea brasiliensis, is a biomaterial that has been studied and has shown promising results in the improvement of regenerative capacity, presenting the capacity to induce neovascularization and tissue proliferation, besides being a material low cost and easy to handle. Another characteristic is the bone graft, such as Beta-Tricalcium phosphate (B-TCP), a phase of calcium phosphate, which has characteristics of biocompatibility, bioactivity, and osteoconductivity. The association of these two characteristics as a possible strategy to optimize bone repair capacity is an alternative to seek better results within a treatment. In short, the purpose of this study is to analyze the influence of B-TCP in conjunction with the natural latex membrane in bone regeneration and remodeling. Fifty (50) Sprague-Dawley rats with a mean weight of 200g will be used. For the induction of bone mass reduction, the rats will be ovariectomized. After 90 days of ovariectomy, a bone defect will be performed bilaterally in the tibia of the animals. The animals will be divided into five experimental groups (n = 10): control group (C), the bone defect will not be treated specifically; Latex (L) group, the bone defect will only be treated with the latex membrane; group (B-TCP), the bone defect will be treated with B-TCP; (L + B-TCP), B-TCP treatment in the bone defect covered with the latex membrane and the SHAM group, in which ovarian removal will not be performed and the bone defects have not received specific treatment. After 21 days, the animals will be euthanized, the tibias and femurs will be dissected and stored. The left tibias and femurs will be used for mechanical assay and bone densitometry, and the right tibias and femurs will be used for histological analysis to characterize bone microarchitecture, quantification of bone neoformation and quantification of collagen fibers.

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