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High throughput screening of potential cytoprotective drugs against oropouche virus infection

Grant number: 19/06459-1
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2020
Effective date (End): February 28, 2022
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal Investigator:Rafael Elias Marques Pereira Silva
Grantee:Alexandre Borin Pereira
Host Institution: Centro Nacional de Pesquisa em Energia e Materiais (CNPEM). Ministério da Ciência, Tecnologia e Inovação (Brasil). Campinas , SP, Brazil

Abstract

Oropouche virus (OROV) is an arbovirus present in the Amazon region and Central America. Oropouche fever has affected more than half million people in the last 50 years, causing fever, joint pain, headache and myalgia. Like other arboviruses, many cases are not diagnosed correctly and there is no specific treatment for the disease. The use of high throughput screening for drug discovery and repurposing of FDA human approved drugs has been shown as an alternative for new treatments, in behalf of high costs and the long time to approve new drugs. The present project aims to stablish and optimize an HTS assay for phenotypic studies using VERO cells infected with OROV, and to select compounds with potential cytoprotection against the infection. Preliminary Results: A stock of OROV was produced from the original strain BeAn 19991 for the HTS assay. In this screening campaign, it was used more than 1000 compounds from the National Institutes of Health Clinical Compound Collection (NIH-NCC) approved for human use. Of those, 2% of the compounds showed a decrease of the death of OROV-infected cells by at least 50%. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
BORIN, ALEXANDRE; COIMBRA, LAIS D.; BISPO-DOS-SANTOS, KARINA; NACIUK, FABRICIO F.; FONTOURA, MARINA; SIMEONI, CAMILA L.; GOMES, GIOVANNI, V; AMORIM, MARIENE R.; GRAVINA, HUMBERTO D.; SHIMIZU, JACQUELINE FARINHA; et al. Identification and characterization of the anti-SARS-CoV-2 activity of cationic amphiphilic steroidal compounds. VIRULENCE, v. 13, n. 1, p. 18-pg., . (20/04558-0, 16/00194-8, 19/06459-1)
BRUNETTI, NATALIA S.; DAVANZO, GUSTAVO G.; DE MORAES, DIOGO; FERRARI, ALLAN J. R.; SOUZA, GABRIELA F.; MURARO, STEFANIE PRIMON; KNITTEL, THIAGO L.; BOLDRINI, VINICIUS O.; MONTEIRO, LAUAR B.; VIRGILIO-DA-SILVA, JOO VICTOR; et al. SARS-CoV-2 uses CD4 to infect T helper lymphocytes. eLIFE, v. 12, p. 26-pg., . (20/04558-0, 15/15626-8, 19/06459-1, 19/04726-2, 19/16116-4, 19/05155-9, 19/13552-8, 16/18031-8, 19/00098-7, 20/04583-4, 16/00194-8, 21/08354-2, 19/17007-4, 19/22398-2, 17/01184-9, 19/14465-1, 20/04579-7, 13/08293-7, 18/14933-2, 17/23920-9, 19/06372-3, 16/24163-4, 16/23328-0, 20/04919-2, 20/04746-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
PEREIRA, Alexandre Borin. Screening of steroidal compounds with potential antiviral activity against SARS-CoV-2 infection. 2022. Master's Dissertation - Universidade Estadual de Campinas (UNICAMP). Instituto de Biologia Campinas, SP.

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