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Antimicrobial activity of proteins secreted by Xanthomonas citri against microorganisms of medical interest

Grant number: 19/21739-0
Support type:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2020
Effective date (End): December 31, 2021
Field of knowledge:Biological Sciences - Microbiology - Applied Microbiology
Principal researcher:Cristiane Rodrigues Guzzo Carvalho
Grantee:Camila Gasque Bomfim
Home Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Xanthomonas citri (XAC) is a phytopathogen that infects citrus trees, causing citrus canker. This bacterium possesses a secretion system that takes part in the competition for the environment, the Type IV Secretion System (T4SS), which is transmembrane machinery that contains an injection pilus responsible for the delivery of the secreted proteins into other microorganisms. One of these proteins, XAC2609, has been described and shown lysozyme-like activity in vitro assays against bacteria. There are other 12 putative effectors in the system, with domains that range from lipase to lysozyme-like and whose structures and functions have not been described yet. Therefore, in this project we aim to define the best methods for expression and purification of two other proteins secreted by the T4SS, XAC2885 and XAC3634, while also establishing protocols for testing the antibacterial activity of three XAC effectors, XAC2609, XAC2885 and XAC3634, and evaluate their effectiveness against microorganisms of clinical interest.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
VENTURA FERNANDES, BIANCA H.; FEITOSA, NATALIA MARTINS; BARBOSA, ANA PAULA; BOMFIM, CAMILA GASQUE; GARNIQUE, ANALI M. B.; ROSA, IVANA F.; RODRIGUES, MAIRA S.; DORETTO, LUCAS B.; COSTA, DANIEL F.; CAMARGO-DOS-SANTOS, BRUNO; et al. oxicity of spike fragments SARS-CoV-2 S protein for zebrafish: A tool to study its hazardous for human health. Science of The Total Environment, v. 813, . (19/00195-2, 18/07098-0, 20/05761-3, 17/17303-7, 20/04680-0, 14/04294-1, 19/21739-0, 19/19939-1, 19/18356-2, 19/14285-3, 19/12234-2)

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