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Evaluation of the therapeutic efficacy of physical activity-associated nanoparticle-labeled stem cells in the focal ischemia model

Grant number: 19/21070-3
Support Opportunities:Scholarships in Brazil - Scientific Initiation
Effective date (Start): January 01, 2020
Effective date (End): December 31, 2020
Field of knowledge:Biological Sciences - Biophysics - Molecular Biophysics
Principal Investigator:Lionel Fernel Gamarra Contreras
Grantee:João Victor Matias Ferreira
Host Institution: Instituto Israelita de Ensino e Pesquisa Albert Einstein (IIEPAE). Sociedade Beneficente Israelita Brasileira Albert Einstein (SBIBAE). São Paulo , SP, Brazil

Abstract

The purpose of this Scientific Initiation project is to evaluate the efficiency of cell therapy combined with physical activity after induction of focal cerebral ischemia using the photothrombosis technique in the animal model. The present study is part of a larger project where we intend to study the cellular and molecular mechanisms in tissue and functional repair through the therapeutic action of bone marrow mesenchymal stem cells (MTC) labeled with multimodal nanoparticles in the animal model with focal ischemia. With the use of nanobiotechnological resources will be possible to monitor these cells in vivo by molecular imaging techniques, enabling the study of cell tracking and migration that will assist in the functional characterization of these cells in the photothrombosis-induced ischemic brain region of the experimental model.Thus, the present project of scientific initiation will be divided into four stages: the first stage will consist of a systematic review based on scientific articles to evaluate the parameters used in the induction of focal cerebral ischemia using the photothrombosis technique. In the second stage will be performed the marking of the MSC with multimodal nanoparticles. The third stage corresponds to the phototrmobesis stroke induction in the animal model. In the fourth stage implantation of the nanoparticle-labeled MSCs in the animal and subsequent evaluation of the MSM homing and tracking. Finally, in the fifth stage will be performed the behavioral assessment and motor activity. (AU)

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Scientific publications (7)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
GARRIGOS, MURILO MONTENEGRO; DE OLIVEIRA, FERNANDO ANSELMO; NUCCI, MARIANA PENTEADO; NUCCI, LEOPOLDO PENTEADO; ALVES, ARIELLY DA HORA; METROPOLO DIAS, OLIVIA FURIAMA; GAMARRA, LIONEL FERNEL. How mesenchymal stem cell cotransplantation with hematopoietic stem cells can improve engraftment in animal models. WORLD JOURNAL OF STEM CELLS, v. 14, n. 8, p. 22-pg., . (16/21470-3, 17/17868-4, 19/21070-3)
THEINEL, MATHEUS H.; NUCCI, MARIANA P.; ALVES, ARIELLY H.; DIAS, OLIVIA F. M.; MAMANI, JAVIER B.; GARRIGOS, MURILO M.; OLIVEIRA, FERNANDO A.; REGO, GABRIEL N. A.; VALLE, NICOLE M. E.; CIANCIARULLO, GABRIELA; et al. The Effects of Omega-3 Polyunsaturated Fatty Acids on Breast Cancer as a Preventive Measure or as an Adjunct to Conventional Treatments. NUTRIENTS, v. 15, n. 6, p. 34-pg., . (16/21470-3, 17/17868-4, 19/21070-3)
GARRIGOS, MURILO M.; OLIVEIRA, FERNANDO A.; NUCCI, MARIANA P.; MAMANI, JAVIER B.; DIAS, OLIVIA F. M.; REGO, GABRIEL N. A.; JUNQUEIRA, MARA S.; COSTA, CICERO J. S.; SILVA, LUCAS R. R.; ALVES, ARIELLY H.; et al. Bioluminescence Imaging and ICP-MS Associated with SPION as a Tool for Hematopoietic Stem and Progenitor Cells Homing and Engraftment Evaluation. PHARMACEUTICS, v. 15, n. 3, p. 18-pg., . (16/21470-3, 17/17868-4, 19/21070-3)
NUCCI, MARIANA P.; OLIVEIRA, FERNANDO A.; FERREIRA, JOAO M.; PINTO, YOLANDA O.; ALVES, ARIELLY H.; MAMANI, JAVIER B.; NUCCI, LEOPOLDO P.; VALLE, NICOLE M. E.; GAMARRA, LIONEL F.. ffect of Cell Therapy and Exercise Training in a Stroke Model, Considering the Cell Track by Molecular Image and Behavioral Analysi. CELLS, v. 11, n. 3, . (16/21470-3, 19/21070-3, 17/17868-4)
NUCCI, MARIANA P.; MAMANI, JAVIER B.; OLIVEIRA, FERNANDO A.; FILGUEIRAS, IGOR S.; ALVES, ARIELLY H.; THEINEL, MATHEUS H.; RODRIGUES, LUIZ D.; MARTI, LUCIANA; GAMARRA, LIONEL F.. Optimization of Multimodal Nanoparticles Internalization Process in Mesenchymal Stem Cells for Cell Therapy Studies. PHARMACEUTICS, v. 14, n. 6, p. 23-pg., . (19/21070-3, 16/21470-3, 17/17868-4)
VALLE, NICOLE M. E.; NUCCI, MARIANA P.; ALVES, ARIELLY H.; RODRIGUES, LUIZ D.; MAMANI, JAVIER B.; OLIVEIRA, FERNANDO A.; LOPES, CAIQUE S.; LOPES, ALEXANDRE T.; CARRENO, MARCELO N. P.; GAMARRA, LIONEL F.. Advances in Concentration Gradient Generation Approaches in a Microfluidic Device for Toxicity Analysis. CELLS, v. 11, n. 19, p. 56-pg., . (16/21470-3, 17/17868-4, 19/21070-3)
ALVES, ARIELLY DA HORA; NUCCI, MARIANA PENTEADO; DO VALLE, NICOLE MASTANDREA ENNES; MISSINA, JULIANA MORAIS; MAMANI, JAVIER BUSTAMANTE; REGO, GABRIEL NERY ALBUQUERQUE; DIAS, OLIVIA FURIAMA METROPOLO; GARRIGOS, MURILO MONTENEGRO; DE OLIVEIRA, FERNANDO ANSELMO; GAMARRA, LIONEL FERNEL. Current overview of induced pluripotent stem cell-based blood-brain barrier-on-a-chip. WORLD JOURNAL OF STEM CELLS, v. 15, n. 6, p. 22-pg., . (16/21470-3, 17/17868-4, 19/21070-3)

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