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Roles of ATPase MINUSCULE in Solanum lycopersicum reproductive development

Grant number: 19/23142-1
Support Opportunities:Scholarships abroad - Research Internship - Post-doctor
Effective date (Start): February 01, 2020
Effective date (End): January 31, 2021
Field of knowledge:Biological Sciences - Genetics - Plant Genetics
Principal Investigator:Fabio Tebaldi Silveira Nogueira
Grantee:Carolina de Marchi Santiago da Silva
Supervisor: Miriam Calonje Macaya
Host Institution: Escola Superior de Agricultura Luiz de Queiroz (ESALQ). Universidade de São Paulo (USP). Piracicaba , SP, Brazil
Research place: Instituto de Bioquímica Vegetal y Fotosíntesis (IBVF), Spain  
Associated to the scholarship:17/26587-9 - Functional characterization of histone deacetylase SlHDA3 in vegetative to reproductive phase transition in tomato., BP.PD


Driving energy from ATP hydrolysis, chromatin may be more accessible to transcription factors. Such chromatin remodeling is achieved by SWI2/SNF2 complex, with a core ATPase BRAHMA (BRM), SPLAYED (SYD) or MINUSCULE (MINU) in terrestrial plants. BRM is the most widely studied in plants, using knock-down mutants in Arabidopsis thaliana to elucidate its function. On the other hand, MINU is until now poorly described, being most phenotypic traits in Arabidopsis MINU knock-out similar to gibberellin physiological effects. Solanum lycopersicum presents different reproductive development when compared to Arabidopsis: opposite flowering pattern in gibberellin pathway and fleshy fruit instead of siliques. The aim of this study is to deeply evaluate functions of SlMINU in tomato reproductive development using for that purpose the RNAi transgenic line we already generated and exhibits promising phenotypes. (AU)

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