Cytocompatibility and effect on osteoblast biology of a new bioceramic material for root canal filling of primary teeth

Abstract

The zinc oxide and eugenol cement (ZOE) and calcium hydroxide paste thickened with zinc oxide are the most frequently used materials for root canal filling of primary teeth. Recently a new bioceramic material for root canal filling of primary teeth, BIO-C Pulpecto (Angelus, Londrina, Paraná, Brazil) has been developed. The aim of this study will be to evaluate the biological properties of cytocompatibility and the potential to induce mineralization, in vitro, of Bio-C Pulpecto compared with ZOE and calcium hydroxide paste (Calen) thickened with zinc oxide. To assess cytocompatibility, Saos-2 (human osteoblast-like cell line) exposed to materials "extracts" studied at different time periods will be subjected to methylthiazole tetrazolium (MTT) and neutral red assays. The potential to induce mineralization will be assessed by the alkaline phosphatase activity and production of mineralized nodules (alizarin red) assays. Statistical tests will be chosen according to the distribution and homoscedasticity of the data employing a significance level of 5%. (AU)