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Evaluation of the proteins differentially expressed in saliva associated with periodontitis during the 3rd trimester of pregnancy in obese and eutrophic patients

Grant number: 18/20626-5
Support type:Scholarships in Brazil - Doctorate
Effective date (Start): December 01, 2019
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal researcher:Silvia Helena de Carvalho Sales Peres
Grantee:Gerson Aparecido Foratori Junior
Home Institution: Faculdade de Odontologia de Bauru (FOB). Universidade de São Paulo (USP). Bauru , SP, Brazil
Associated scholarship(s):19/26845-3 - Systemic and oral evaluations and metabolomic profiling of the saliva and plasma by quantitative proton nuclear magnetic resonance (1H-NMR) in obese and eutrophic pregnant women, BE.EP.DR


Obesity and pregnancy are factors in which systemic and oral changes can be noticed. The adipose tissue of obese patients secretes inflammatory mediators that reduce the immune response of the host and exacerbate the inflammatory response. The mechanism during pregnancy is similar, due to estrogen and progesterone levels alterations. Therefore, both Obesity and pregnancy are associated with high inflammation in periodontal tissues. Salivary proteome analysis is a method for the large-scale identification of proteins and biomarkers, which can be used to diagnose local and systemic diseases. The aim of this study will be to identify the proteins differentially expressed in saliva associated with periodontitis during the third trimester of gestation in obese and eutrophic patients. The sample will be consisted by 40 pregnant women divided into: GOP - Obese with periodontitis (n=10); GOWP - Obese without periodontitis (n=10); GEP - Eutrophic with periodontitis (n=10); and GEWP - Eutrophic without periodontitis (n=10). Grouping sample will occur according to anthropometric parameters - weight, height and Body Mass Index (BMI); and periodontal parameters - Periodontal Pocket Depth (PPD) and Clinical Attachment Level (CAL). Periodontitis will be considered from the presence of 2 or more interproximal sites with CAL e 3 mm and 2 or more interproximal sites of different teeth with PPD e 4 mm or 1 interproximal site with PPD e 5 mm. For proteomics salivary analysis, the sample will be evaluated individually and they will be processed for analysis by Nano Liquid Chromatography Electron Spray Ionization Tandem Mass Spectrometry (nLC-ESI-MS/MS). The obtained MS/MS spectra will be compared to human protein databases (UniProtKB/Swiss-Prot). Free marker quantification will be performed using Protein Lynx Global Service (PLGS) software. For the comparison of the anthropometric and periodontal parameters, the Kolmogorov-Smirnov test will be applied to evaluate the normality of the quantitative variables (weight, height, BMI, PPD and CAL). ANOVA will be applied for the quantitative variables with normal distribution and Kruskal-Wallis for the quantitative variables without normality. The level of significance of 5% will be adopted. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FORATORI-JUNIOR, GERSON APARECIDO; MOSQUIM, VICTOR; RABELO BUZALAF, MARILIA AFONSO; DE CARVALHO SALES-PERES, SILVIA HELENA. Salivary cytokines levels, maternal periodontitis and infants' weight at birth: A cohort study in pregnant women with obesity. Placenta, v. 115, p. 151-157, NOV 2021. Web of Science Citations: 0.
FORATORI-JUNIOR, GERSON APARECIDO; TRENHAGO MISSIO, ALANA LUIZA; ORENHA, ELIEL SOARES; DE CARVALHO SALES-PERES, SILVIA HELENA. Systemic Condition, Periodontal Status, and Quality of Life in Obese Women During Pregnancy and After Delivery. INTERNATIONAL DENTAL JOURNAL, v. 71, n. 5, p. 420-428, OCT 2021. Web of Science Citations: 0.
JESUINO, BRUNO GUALTIERI; FORATORI-JUNIOR, GERSON APARECIDO; MISSIO, ALANA LUIZA TRENHAGO; MASCOLI, LEONARDO SILVA; SALES-PERES, SILVIA HELENA DE CARVALHO. Periodontal status of women with excessive gestational weight gain and the association with their newborns' health. INTERNATIONAL DENTAL JOURNAL, v. 70, n. 5 JUN 2020. Web of Science Citations: 0.

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