Genome-wide approaches to reveal epigenomic structure in trypanosomes subjected to metabolic states changes and during cell cycle

Abstract

Parasites submitted to different metabolic backgrounds, such as severe metabolic stress or metabolic stress conditions in the presence of single carbon and energy sources for maintenance (PBS supplemented with glucose, proline, histidine or leucine) could have different epigenomes. These differences could affect cell cycle, differentiation or host-cell invasion among other processes. In this proposal, we will evaluate the epigenome of parasites maintained under different metabolic conditions. We will take advantage of two genome-wide methods that allow the identification of nucleosomes position as well as putative regulatory sequences, possibly associated with highly active transcribed regions. These two approaches will be complementary and will make it possible to discriminate unbiased important genomic regions associated either with metabolic states or cell cycle.To this end, an intense bioinformatic approach to integrate these datasets as well as to explore functional annotation available at public datasets will also be implemented. In addition, comparison between T.cruzi and Leishmania data will be used as a valuable resource to better understand similarities and differences inherent to their biology. (AU)