Candida spp. is responsible for over 80% of cases of nosocomial fungal infection, representing an important cause of morbidity and mortality. Among the Candida species, Candida parapsilosis has been the most prevalent in neonatal intensive care units. The ability of this pathogen to form biofilm and colonize medical devices and hands of health professionals favors its horizontal transmission and the occurrence of hospital outbreaks. Several studies have revealed the genetic similarity between the clinical isolates and isolates obtained from hands of these professionals. Therefore, investigations on the molecular epidemiology of C. parapsilosis (sensu stricto), predominant species of C. parapsilosis complex, have been performed. However, low discriminatory methodologies such as PFGE and RAPD have been used for molecular typing. Thus, the employment of more discriminating genotyping methods is highly relevant for elucidating the dispersion and persistence of this yeast in the hospital environment. The present study aims to determine the genotypes of C. parapsilosis (sensu stricto) clinical isolates in a large tertiary Brazilian hospital by microsatellite analysis, which is one of the main molecular typing tools used in investigations of hospital outbreaks of candidemia.
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