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Influence of L-arginine and polyamines metabolism for transcripts and microRNAs profile in murine macrophages infected with Leishmania amazonensis

Grant number: 19/07089-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): October 01, 2019
Effective date (End): February 28, 2022
Field of knowledge:Biological Sciences - Parasitology
Principal Investigator:Sandra Marcia Muxel
Grantee:Jonathan Miguel Zanatta
Host Institution: Instituto de Biociências (IB). Universidade de São Paulo (USP). São Paulo , SP, Brazil


Mammalian host infected with protozoan parasite Leishmania amazonensis causes a cutaneous leishmaniosis, a neglected disease that present a higher prevalence in Brazil. The establishment of immunological response uses post-transcriptional resources, such as microRNAs, which are largely studied in leishmaniasis. The ways to forward the control of gene expression regulation from polyamines pathway and production of nitric oxide (NO) have been studied and guides a minucious regulatory mechanism mediated by microRNAs (miRNAs). Indeed, both parasite and macrophages use L-arginine as substrate for polyamines pathway via arginase enzyme; or NO via nitric oxide synthase; and thus modulating the balance between the survival of parasite and the establishment of inflammatory response. Thus, the combined efforts to understand the perturbation promoted by Leishmania into the host cells, evidenced the fundamental importance of the L-arginine metabolism to maintain the infection. In this way, the present proposal aims to analyze the expression of mRNAs and miRNAs involved in the L-arginine metabolism in macrophages of BALB/c mice infected with L. amazonensis, under conditions of deprivation or supplementation of L-arginine and putrescine and how these metabolites influences the infectivity, allowing or blocking parasite replication.

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ZANATTA, JONATHAN MIGUEL; ACUNA, STEPHANIE MAIA; ANGELO, YAN DE SOUZA; BENTO, CAMILLA DE ALMEIDA; PERON, JEAN PIERRE SCHATZMAN; STOLF, BEATRIZ SIMONSEN; MUXEL, SANDRA MARCIA. Putrescine supplementation shifts macrophage L-arginine metabolism related-genes reducing Leishmania amazonensis infection. PLoS One, v. 18, n. 3, p. 25-pg., . (17/23519-2, 22/00291-4, 18/24693-9, 18/18499-5, 19/07089-3)
ACUNA, STEPHANIE MAIA; ZANATTA, JONATHAN MIGUEL; DE ALMEIDA BENTO, CAMILLA; FLOETER-WINTER, LUCILE MARIA; MUXEL, SANDRA MARCIA. iR-294 and miR-410 Negatively Regulate Tnfa, Arginine Transporter Cat1/2, and Nos2 mRNAs in Murine Macrophages Infected with Leishmania amazonensi. ON-CODING RN, v. 8, n. 1, . (18/24693-9, 19/07089-3, 18/18499-5, 17/23519-2, 14/50717-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ZANATTA, Jonathan Miguel. Polyamines regulate the expression of genes related to its metabolism and resistance to infection of macrophages with Leishmania amazonensis. 2022. Master's Dissertation - Universidade de São Paulo (USP). Instituto de Biociências (IBIOC/SB) São Paulo.

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