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Fertility effects of antioxidants and AI timing with cryopreserved pig sperm used in a single fixed time insemination

Grant number: 19/15668-3
Support type:Scholarships abroad - Research
Effective date (Start): December 03, 2019
Effective date (End): December 02, 2020
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal researcher:André Furugen Cesar de Andrade
Grantee:André Furugen Cesar de Andrade
Host: Robert Knox
Home Institution: Faculdade de Medicina Veterinária e Zootecnia (FMVZ). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Research place: University of Illinois at Urbana-Champaign, United States  

Abstract

The aim of this work will be to evaluate how supplementing freezing media with reduced glutathione (GSH) or BHT affects in-vitro sperm fertility and its impact when a single insemination is performed at different intervals before ovulation. Experiment 1- In total 5 healthy boars from the Landrace x Largewhite, 2-3 years age will be used in this study. Ejaculates will be collected and pooled. Thus, there will be, at least, five replicates per treatment. The treatments will be: 1) cryopreserved semen without antioxidants (Control), 2) cryopreserved semen with 1 mM BHT and 3) cryopreserved semen with 2mM GSH. All semen evaluation will be performed at 30, and 240 min after thawing. The analysis will consist of CASA, sperm membrane integrity, membrane fluidity, lipid peroxidation and superoxide anion production. In Experiment 2- Gilts will be induced into estrus with PG600 and then estrus synchronized by feeding 15 mg×gilt×d of Matrix for 14 d as a top-dress on a standard sow gestation diet. Gilts will be treated with 200µg Tripitorelin (OvuGel) 130 hours of the last Matrix feeding to synchronize ovulation. Gilts (n=20/treatment) will be inseminated at 20 or 30 hours after OvuGel with a single fixed time artificial insemination with 2 x 109 cryopreserved sperm without antioxidants (Control), cryopreserved semen with 1 mM BHT and cryopreserved semen with 2mM GSH. Gilts will be slaughtered between days 28 and 34 of gestation and reproductive tracts collected. The tracts will be identified and processed for pregnancy status, number of normal fetuses, number of degenerative fetuses, number of corpora lutea (CL). Continuous response variables will be analyzed using MIXED and binary response variables analyzed using the GENMOD procedures of SAS (SAS Institute Inc., Cary, NC).

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