The protector effect of hidratation in contrast induced nephropathy in diabetic rats

Abstract

Acute kidney injury (AKI) is characterized by increased serum creatinine and urinary output in 48 hours after clinical, surgical and infectious events. Among the causes of AKI is contrast-induced nephropathy (CIN), which has diabetes mellitus as a predisposing factor. Objective: To verify the effect of saline hydration on renal function, renal hemodynamics and on the oxidative profile of CIN in diabetic rats. Methods: 28 Wistar rats, male and adult, weighing between 250-290g will be used. The animals will be randomized into the following experimental groups: a) Citrate: animals that will receive the citrate buffer at pH 4.2, intravenous (i.v.); b) Diabetes (DM): animals receiving 65 mg/kg of streptozotocin, i.v., diluted in 0.1 M citrate buffer at pH 4.2 on the 1st day of protocol and will continue to be followed up until the 28th day of the experimental protocol; c) Diabetes + Iodinated contrast (DM + CI): DM animals receiving 6 ml / kg IC, intraperitoneal (i.p.), single dose on the 26th day of the experimental protocol; d) Diabetes + Iodinated Contrast + Saline Hydration (DM + CI + H): DM + CI animals that will receive 12ml / kg of NaCl 0.9% i.p. on the 23rd day, for five days. Physiological parameters such as weight, feed intake and water, glycemia and kidney weight and animal weight ratio will be evaluated; renal function (clearance of inulin and creatinine); renal hemodynamics and oxidative profile (urinary peroxides and TBARS, renal tissue thiols and urinary nitric oxide). The results will be expressed as mean ± standard deviation. The variance between the groups will be analyzed using the One Way ANOVA test, followed by the Newman-Keuls multiple-comparator post-test of the Graph-Pad Prism version-3 for Windows® statistical program. Values of p <0.05 will be considered significant. Expected results: Based on data analysis, the present investigation may contribute to elucidate whether pretreatment with saline hydration represents a therapeutic alternative in the prevention of CIN.