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Byproducts recovery from carrot juice processing: extraction of phytochemical compounds, encapsulation and application in biodegradable films

Grant number: 19/05511-0
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): June 01, 2019
Effective date (End): August 31, 2021
Field of knowledge:Agronomical Sciences - Food Science and Technology - Food Engineering
Principal Investigator:Milena Martelli Tosi
Grantee:Bruno Stefani Esposto
Host Institution: Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (FFCLRP). Universidade de São Paulo (USP). Ribeirão Preto , SP, Brazil
Associated scholarship(s):19/22502-4 - Study of in vitro digestion of TPP-chitosomes encapsulating phytochemical compounds extracted from carrot byproducts, BE.EP.MS


This project consists in optimizing the extraction of phytochemical compounds from byproducts obtained after carrot (Daucus carota) juice processing, encapsulate the compounds and then incorporate them into chitosan biodegradable films. Thus, three steps will be followed: (i) initially, an experimental design (22) will be performed to optimize the extraction of phenolic compounds and carotenoids from the byproducts, using ethanol or vegetal oil concentration, respectively, and potency/time of ultrasound sonicator as independent variables. The quantification of total phenolic compounds will be performed by spectrophotometry and the identification of the compounds in the optimum condition will be evaluated by UPLC-MS. The quantification of carotenoids will be performed with the aid of HPLC; (ii) the active compounds present in the extracts obtained in the optimal condition will be encapsulated in quitosomes, hybrid systems composed by liposomes covered with chitosan, using sodium tripolyphosphate for ionotropic gelation. These samples will be analyzed according to particle size by dynamic light scattering and surface charge by zeta potential, Attenuated Total Reflection/Fourier Transform Infrared Spectroscopy, Raman microspectrometry and morphology (Confocal microscopy and Field Emission Scanning Electron Microscopy); (iii) lastly, the encapsulate compounds with better stability will be incorporated into chitosan biodegradable films. The films will have their mechanical and function properties analyzed and the release of phytochemicals added to the polymeric matrix will be evaluated. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
ESPOSTO, BRUNO STEFANI; BOTELHO PINHO, SABRINA GUARDA; THOMAZINI, MARCELO; RAMOS, ANA PAULA; TAPIA-BLACIDO, DELIA RITA; MARTELLI-TOSI, MILENA. TPP-chitosomes as potential encapsulation system to protect carotenoid-rich extract obtained from carrot by-product: A comparison with liposomes and chitosomes. Food Chemistry, v. 397, p. 8-pg., . (19/22502-4, 19/05511-0, 16/18788-1, 19/23171-1)
ESPOSTO, BRUNO STEFANI; JAUREGI, PAULA; TAPIA-BLACIDO, DELIA R.; MARTELLI-TOSI, MILENA. Liposomes vs. chitosomes: Encapsulating food bioactives. TRENDS IN FOOD SCIENCE & TECHNOLOGY, v. 108, p. 40-48, . (19/22502-4, 19/23171-1, 19/05511-0, 16/18788-1)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
ESPOSTO, Bruno Stefani. By-product recovery from carrot juice processing: extraction of phytochemical compounds, encapsulation and application in biodegradable films. 2021. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto (PCARP/BC) Ribeirão Preto.

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