Endocrinology is a widely used tool for the conservation of endangered species, providing information about the physiology and reproductive status of animals through the measurement of hormones, mainly from the fecal matrix. However, several studies have shown that the precision of this method can vary according to the amount of dietary fiber in the animals, influencing the excretion of fecal metabolites. This study aims to evaluate whether dietary fiber affects the excretion of fecal androgen metabolites. To that end, four males of the species Mazama gouazoubira, housed in the Núcleo de Pesquisa e Conservação de Cervídeos, UNESP/Jaboticabal (NUPECCE), will be submitted to four treatments, distributed among the animals by the Latin square method: usual feeding provided in NUPECCE (T1); 1kg/animal/day of bulky and 500g/animal/day of banana (T2); 1.5kg/animal/day of banana (T3); 1.5 kg/animal/day of bulky (T4). Each treatment will last six days, the animals will have an adaptation period of three days, and during the next three days, feces will be collected (in the morning and afternoon, before feeding). The treatments will be carried out in sequence, with no rest time for between them. Metabolite levels will be measured using the immunoenzymatic assay, and then the concentration means will be compared between the different treatments through the execution of Student's t-test.
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