Generation and characterization of gemcitabine-resistant pancreatic carcinoma-derived cell lines

Abstract

The project aims to generate immortalized PDAC cell lines resistant to gemcitabine, and the molecular characterization of this resistance. Such lines will be generated by prolonged treatments under sub-lethal concentrations of the drug and will have their resistance evaluated by determining the expression of resistance marker genes already described in the literature by RT-qPCR. In addition to widely studied PDAC lines, a line derived from PDAC xenotransplantation will also be used, which more reliably recapitulates the patient's tumor microenvironment. The results obtained in this project will be the basis for a project under development in the Laboratory of Integrated Research in Cancer of the IQ-USP, which aims to perform the scan of components of the epigenetic machinery functionally associated with chemoresistance and sensitivity to gemcitabine in cancer of pancreas.