The osseointegration of titanium (Ti) implants is based on bone remodeling, which involves a balance between bone tissue formation by osteoblasts and tissue resorption by osteoclasts. Our group has shown that a Ti surface with nanotopography obtained by chemical conditioning with H2SO4/H2O2 solution is capable of inducing osteoblast differentiation under both osteogenic and non-osteogenic conditions. However, the effect of this surface on osteoclasts, and their interaction with osteoblasts have not been investigated yet. To understand the transcriptional changes caused by Ti surface with nanotopography during the interaction between osteoblastic and osteoclastic cells, we will evaluate by high-throughput RNA sequencing: (1) the effect of Ti with nanotopography surface (Ti-Nano), compared with control Ti surface, on osteoblastic and osteoclastic differentiation in isolated cultures of MC3T3-E1 and RAW 264.7 cells, respectively, grown on these surfaces, (2) the effect of MC3T3-E1 cells on osteoclastic differentiation of RAW 264.7 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models and (3) the effect of RAW 264.7 cells on osteoblastic differentiation of MC3T3-E1 cells grown on Ti surface with nanotopography, compared with control Ti surface, in indirect co-culture models. Differential expression analysis will be performed with DESeq2 considering fold change >2 to differential gene expression, and p-value <0.05.
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