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UCP1-independent mechanisms involved in the increased energy expenditure and protection against Obesity induced by fish oil in mice

Grant number: 19/04271-5
Support Opportunities:Scholarships in Brazil - Doctorate
Effective date (Start): June 01, 2019
Effective date (End): October 31, 2022
Field of knowledge:Biological Sciences - Physiology - Physiology of Organs and Systems
Principal Investigator:William Tadeu Lara Festuccia
Grantee:Tiago Eugênio Oliveira da Silva
Host Institution: Instituto de Ciências Biomédicas (ICB). Universidade de São Paulo (USP). São Paulo , SP, Brazil
Associated research grant:15/19530-5 - Involvement of the nutrient sensor mTOR in the development of obesity associated chronic metabolic diseases, AP.TEM


Intake of a high fat diet rich in fish oil and omega 3 fatty acids (n-3 fatty acids) protects mice from body weight gain and Obesity by increasing energy expenditure in an UCP1-independent manner. We will investigate herein the involvement skeletal muscle, heart and liver peroxisomal and mitochondrial oxidative metabolism, as well as SERCA-mediated calcium cycling as mediators of the increased in energy expenditure induced by intake of a high fat diet rich in fish oil. For this, wild type and UCP1 KO mice will be fed for 8 weeks with high fat diet produced using either lard (HFD) or fish oil (HFN3) as fat source and evaluated for body weight, adiposity, indirect calorimetry, body temperature, mitochondrial function, expression and content of enzymes involved in peroxisomal and mitochondrial beta-oxidation and biogenesis. In a second protocol, these parameters will be evaluated in wild type and UCP1 KO mice fed during 4 weeks with either HFD or HFN3 and treated with the pharmacological inhibitor of acyl-CoA oxidase (ACOX-1) 10,12-tricosadiynoic acid (100 mg/kg, gavagem). In a third protocol, these parameters will be evaluated in controls and in mice with PEX-5 deficiency exclusively in hepatocytes. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CASTRO, ERIQUE; VIEIRA, THAYNA S.; OLIVEIRA, TIAGO E.; ORTIZ-SILVA, MILENE; ANDRADE, MAYNARA L.; TOMAZELLI, CAROLINE A.; PEIXOTO, ALBERT S.; SOBRINHO, CLEYTON R.; MORENO, MAYARA F.; GILIO, GUSTAVO R.; et al. Adipocyte-specific mTORC2 deficiency impairs BAT and iWAT thermogenic capacity without affecting glucose uptake and energy expenditure in cold-acclimated mice. AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, v. 321, n. 5, p. E592-E605, . (16/23169-9, 19/25943-1, 17/23040-9, 15/13508-8, 17/17582-3, 18/03418-0, 17/17403-1, 17/12260-8, 19/01763-4, 19/17660-0, 15/19530-5, 20/09399-7, 19/04271-5, 12/25317-4)
ANDRADE, MAYNARA L.; GILIO, GUSTAVO R.; PERANDINI, LUIZ A.; PEIXOTO, ALBERT S.; MORENO, MAYARA F.; CASTRO, ERIQUE; OLIVEIRA, TIAGO E.; VIEIRA, THAYNA S.; ORTIZ-SILVA, MILENE; THOMAZELLI, CAROLINE A.; et al. PAR gamma-induced upregulation of subcutaneous fat adiponectin secretion, glyceroneogenesis and BCAA oxidation requires mTORC1 activit. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS, v. 1866, n. 8, . (15/19530-5, 17/17582-3, 13/07937-8, 19/01763-4, 17/12260-8, 15/22983-1, 16/23169-9, 19/04271-5, 15/13508-8, 18/11156-5, 19/17660-0, 17/23040-9, 17/17403-1)

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