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Development of 3-dimensional (3D) printed electrochemical biosensors with PLA polymer and graphene for the determination of biomolecules and diagnosis of diseases

Grant number: 19/00473-2
Support Opportunities:Scholarships in Brazil - Post-Doctoral
Effective date (Start): May 01, 2019
Effective date (End): June 30, 2023
Field of knowledge:Physical Sciences and Mathematics - Chemistry - Inorganic Chemistry
Principal Investigator:Juliano Alves Bonacin
Grantee:Cristiane Kalinke
Host Institution: Instituto de Química (IQ). Universidade Estadual de Campinas (UNICAMP). Campinas , SP, Brazil
Associated research grant:13/22127-2 - Development of novel materials strategic for integrated analytical devices, AP.TEM
Associated scholarship(s):21/07989-4 - Additive-manufactured (3D printed) electrochemical devices for the diagnosis of viral diseases, BE.EP.PD

Abstract

Electrochemical sensors and biosensors can enable disease diagnosis in a fast and accurate manner, using easy manufacturing and handle devices. The need to use new methodologies for the detection of diseases makes the development of these sensors viable. The electrochemical techniques also present some advantages as the low instrumental cost and high sensitivity, allowing the detectability of species in biological samples, with accurate and reliable results in short time periods, compared to other conventional methods used for the detection of diseases. Thus, using PLA polymer and graphene, we intend to make 3D printed electrodes and apply in the determination of biomolecules/biomarkers related to the presence of allergies and diseases. The biosensors will be used in the development of alternative analytical methodologies, by the incorporation of antibodies for the detection of BSA (Bovine Serum Albumin), related to allergies caused by cow milk; and IgG antigen for detection of Yellow Fever virus. Furthermore, it is intended to carry out the electrochemical detection of biomarkers related to Alzheimer's Disease. It is expected to obtain sensitive, selective and robust devices, which present high detectability for these species. (AU)

Articles published in Pesquisa para Inovação FAPESP about the scholarships:
Low-cost sensor detects early-stage Parkinson?s disease in biological samples 
News published in Agência FAPESP Newsletter about the scholarship:
Articles published in other media outlets (0 total):
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Scientific publications (25)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CAMARGO, JESSICA ROCHA; ORZARI, LUIZ OTAVIO; ARAUJO, DIELE APARECIDA GOUVEIA; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; ROCHA, DIEGO PESSOA; DOS SANTOS, ANDRE LUIZ; TAKEUCHI, REGINA MASSAKO; MUNOZ, RODRIGO ALEJANDRO ABARZA; BONACIN, JULIANO ALVES; et al. Development of conductive inks for electrochemical sensors and biosensors. Microchemical Journal, v. 164, . (17/21097-3, 19/23342-0, 13/22127-2, 19/23177-0, 19/00473-2, 17/23960-0, 19/01844-4)
BRAZACA, LAIS CANNIATTI; DOS SANTOS, PAMYLA LAYENE; DE OLIVEIRA, PAULO ROBERTO; ROCHA, DIEGO PESSOA; STEFANO, JESSICA SANTOS; KALINKE, CRISTIANE; MUNOZ, RODRIGO ALEJANDRO ABARZA; BONACIN, JULIANO ALVES; JANEGITZ, BRUNO CAMPOS; CARRILHO, EMANUEL. Biosensing strategies for the electrochemical detection of viruses and viral diseases e A review. Analytica Chimica Acta, v. 1159, . (18/19750-3, 19/00473-2, 13/22127-2, 19/01844-4, 17/21097-3)
BLASQUES, RODRIGO VIEIRA; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; BRAZACA, LAIS CANNIATTI; CRAPNELL, ROBERT D. D.; BONACIN, JULIANO ALVES; BANKS, CRAIG E. E.; JANEGITZ, BRUNO CAMPOS. Flexible Label-Free Platinum and Bio-PET-Based Immunosensor for the Detection of SARS-CoV-2. BIOSENSORS-BASEL, v. 13, n. 2, p. 19-pg., . (19/00473-2, 17/21097-3, 18/19750-3, 19/23177-0, 21/07989-4, 13/22127-2)
KALINKE, CRISTIANE; NEUMSTEIR, NAILE VACIONOTTO; DE OLIVEIRA, PAULO ROBERTO; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. Sensing of L-methionine in biological samples through fully 3D-printed electrodes. Analytica Chimica Acta, v. 1142, p. 135-142, . (17/23960-0, 19/00473-2, 17/21097-3, 19/01844-4, 13/22127-2)
DOS SANTOS, VANESSA W.; MARTINS, GUSTAVO; GOGOLA, JEFERSON L.; KALINKE, CRISTIANE; AGUSTINI, DEONIR; BERGAMINI, MARCIO F.; MARCOLINO-JUNIOR, LUIZ H.. Simultaneous detection of dopamine and ascorbic acid by using a thread-based microfluidic device and multiple pulse amperometry. ANALYTICAL METHODS, v. 15, n. 37, p. 8-pg., . (19/00473-2)
JANEGITZ, BRUNO C.; CRAPNELL, ROBERT D.; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; WHITTINGHAM, MATTHEW J.; FERRARI, ALEJANDRO GARCIA-MIRANDA; BANKS, CRAIG E.. Novel Additive Manufactured Multielectrode Electrochemical Cell with Honeycomb Inspired Design for the Detection of Methyl Parathion in Honey Samples. ACS MEASUREMENT SCIENCE AU, v. 3, n. 3, p. 9-pg., . (17/21097-3, 21/07989-4, 22/01601-7, 19/00473-2)
CRAPNELL, ROBERT D.; SIGLEY, EVELYN; WILLIAMS, RHYS J.; BRINE, TOM; GARCIA-MIRANDA FERRARI, ALEJANDRO; KALINKE, CRISTIANE; JANEGITZ, BRUNO C.; BONACIN, JULIANO A.; BANKS, CRAIG E.. Circular Economy Electrochemistry: Recycling Old Mixed Material Additively Manufactured Sensors into New Electroanalytical Sensing Platforms. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 11, n. 24, p. 11-pg., . (17/21097-3, 21/07989-4, 19/00473-2, 13/22127-2)
CRAPNELL, ROBERT D.; ARANTES, IANA V. S.; WHITTINGHAM, MATTHEW J.; SIGLEY, EVELYN; KALINKE, CRISTIANE; JANEGITZ, BRUNO C.; BONACIN, JULIANO A.; PAIXAO, THIAGO R. L. C.; BANKS, CRAIG E.. Utilising bio-based plasticiser castor oil and recycled PLA for the production of conductive additive manufacturing feedstock and detection of bisphenol A. GREEN CHEMISTRY, v. 25, n. 14, p. 10-pg., . (19/00473-2, 22/07552-8, 17/21097-3, 18/08782-1, 21/07989-4, 19/15065-7, 13/22127-2)
KALINKE, CRISTIANE; DE OLIVEIRA, PAULO ROBERTO; BANKS, CRAIG E.; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. 3D-printed immunosensor for the diagnosis of Parkinson?s disease. SENSORS AND ACTUATORS B-CHEMICAL, v. 381, p. 8-pg., . (19/01844-4, 19/00473-2, 17/21097-3, 21/07989-4, 13/22127-2)
DE OLIVEIRA, PAULO ROBERTO; CRAPNELL, ROBERT D.; FERRARI, ALEJANDRO GARCIA -MIRANDA; WUAMPRAKHON, PHATSAWIT; HURST, NICHOLAS J.; DEMPSEY-HIBBERT, NINA C.; SAWANGPHRUK, MONTREE; JANEGITZ, BRUNO CAMPOS; BANKS, CRAIG E.. Low-cost, facile droplet modification of screen-printed arrays for internally validated electrochemical detection of serum procalcitonin. BIOSENSORS & BIOELECTRONICS, v. 228, p. 9-pg., . (17/21097-3, 21/07989-4, 22/01601-7, 19/00473-2)
FEDALTO, LUCAS; DE OLIVEIRA, PAULO ROBERTO; AGUSTINI, DEONIR; KALINKE, CRISTIANE; BANKS, CRAIG E.; BERGAMINI, MARCIO F.; MARCOLINO-JUNIOR, LUIZ HUMBERTO. Novel and highly stable strategy for the development of microfluidic enzymatic assays based on the immobilization of horseradish peroxidase (HRP) into cotton threads. Talanta, v. 252, p. 6-pg., . (19/00473-2)
DA SILVA, VINICIUS A. O. P.; STEFANO, JESSICA S.; KALINKE, CRISTIANE; BONACIN, JULIANO. A. A.; JANEGITZ, BRUNO. C. C.. Additive Manufacturing Sensor for Stress Biomarker Detection. CHEMOSENSORS, v. 11, n. 5, p. 14-pg., . (22/06145-0, 19/00473-2, 17/21097-3, 21/03179-8, 21/07989-4)
KALINKE, CRISTIANE; CRAPNELL, ROBERT D.; SIGLEY, EVELYN; WHITTINGHAM, MATTHEW J.; DE OLIVEIRA, PAULO R.; BRAZACA, LAIS C.; JANEGITZ, BRUNO C.; BONACIN, JULIANO A.; BANKS, CRAIG E.. Recycled additive manufacturing feedstocks with carboxylated multi-walled carbon nanotubes toward the detection of yellow fever virus cDNA. CHEMICAL ENGINEERING JOURNAL, v. 467, p. 10-pg., . (19/01844-4, 19/00473-2, 17/21097-3, 21/07989-4, 13/22127-2)
OLIVEIRA, PAULO ROBERTO; GUTERRES E SILVA, LUIZ RICARDO; KALINKE, CRISTIANE; FERRARI, ALEJANDRO GARCIA-MIRANDA; PRAKASH, JAI; WEN, YANGPING; NOCELLI, ROBERTA CORNELIO FERREIRA; BONACIN, JULIANO ALVES; BANKS, CRAIG E.; JANEGITZ, BRUNO CAMPOS. Conductive Biofilm Propolis-Based: Electrochemical Determination of Hydroxymethylfurfural in Honey. FOOD ANALYTICAL METHODS, v. N/A, p. 10-pg., . (19/01844-4, 19/00473-2, 17/21097-3, 22/01601-7, 21/07989-4, 13/22127-2)
SIGLEY, EVELYN; KALINKE, CRISTIANE; CRAPNELL, ROBERT D.; WHITTINGHAM, MATTHEW J.; WILLIAMS, RHYS J.; KEEFE, EDMUND M.; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES; BANKS, CRAIG E.. Circular Economy Electrochemistry: Creating Additive Manufacturing Feedstocks for Caffeine Detection from Post- Industrial Coffee Pod Waste. ACS SUSTAINABLE CHEMISTRY & ENGINEERING, v. 11, n. 7, p. 11-pg., . (19/01844-4, 19/00473-2, 17/21097-3, 21/07989-4, 13/22127-2)
DOS SANTOS, VANESSA W.; MARTINS, GUSTAVO; GOGOLA, JEFERSON L.; KALINKE, CRISTIANE; AGUSTINI, DEONIR; BERGAMINI, MARCIO F.; MARCOLINO-JUNIOR, LUIZ H.. Simultaneous detection of dopamine and ascorbic acid by using a thread-based microfluidic device and multiple pulse amperometry. ANALYTICAL METHODS, v. N/A, p. 8-pg., . (19/00473-2)
KALINKE, CRISTIANE; NEUMSTEIR, NAILE VACILOTTO; APARECIDO, GABRIEL DE OLIVEIRA; DE BARROS FERRAZ, THIAGO VASCONCELOS; DOS SANTOS, PAMYLA LAYENE; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. Comparison of activation processes for 3D printed PLA-graphene electrodes: electrochemical properties and application for sensing of dopamine. ANALYST, v. 145, n. 4, p. 1207-1218, . (17/23960-0, 17/21898-6, 17/21097-3, 19/00473-2, 13/22127-2)
VINICIUS A. O. P. DA SILVA; VINICIUS A. P. TARTARE; CRISTIANE KALINKE; PAULO ROBERTO DE OLIVEIRA; DANIEL CARDOSO DE SOUZA; JULIANO A. BONACIN; BRUNO C. JANEGITZ. CONSTRUÇÃO DE UM SUPORTE AJUSTÁVEL LAB-MADE IMPRESSO EM 3D PARA MEDIÇÃO DE ÂNGULO DE CONTATO. Química Nova, v. 43, n. 9, p. 1312-1319, . (19/00473-2, 17/21097-3, 19/01844-4, 13/22127-2, 17/23960-0)
CAMARGO, JESSICA R.; ANDREOTTI, ISABELLA A. A.; KALINKE, CRISTIANE; HENRIQUE, JULIA M.; BONACIN, JULIANO A.; JANEGITZ, BRUNO C.. Waterproof paper as a new substrate to construct a disposable sensor for the, electrochemical determination of paracetamol and melatonin. Talanta, v. 208, . (17/21898-6, 18/02162-1, 17/21097-3, 19/00473-2, 13/22127-2)
CARDOSO, RAFAEL M.; KALINKE, CRISTIANE; ROCHA, RAQUEL G.; DOS SANTOS, PAMYLA L.; ROCHA, DIEGO P.; OLIVEIRA, PAULO R.; JANEGITZ, BRUNO C.; BONACIN, JULIANO A.; RICHTER, EDUARDO M.; MUNOZ, RODRIGO A. A.. Additive-manufactured (3D-printed) electrochemical sensors: A critical review. Analytica Chimica Acta, v. 1118, p. 73-91, . (19/01844-4, 17/23960-0, 13/22127-2, 19/00473-2)
KALINKE, CRISTIANE; DE OLIVEIRA, PAULO ROBERTO; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. Prussian blue nanoparticles anchored on activated 3D printed sensor for the detection of L-cysteine. SENSORS AND ACTUATORS B-CHEMICAL, v. 362, p. 9-pg., . (19/00473-2, 19/01844-4, 17/21097-3, 13/22127-2)
KALINKE, CRISTIANE; DE OLIVEIRA, PAULO ROBERTO; NEUMSTEIR, NAILE VACILOTTO; HENRIQUES, BRUNNA FERRI; APARECIDO, GABRIEL DE OLIVEIRA; LOUREIRO, HUGO CAMPOS; JANEGITZ, BRUNO CAMPOS; BONACIN, JULIANO ALVES. Influence of filament aging and conductive additive in 3D printed sensors. Analytica Chimica Acta, v. 1191, . (19/00473-2, 17/21097-3, 19/01844-4, 13/22127-2)
KALINKE, CRISTIANE; DE OLIVEIRA, PAULO R.; BONACIN, JULIANO A.; JANEGITZ, BRUNO C.; MANGRICH, ANTONIO S.; MARCOLINO-JUNIOR, LUIZ H.; BERGAMINI, MARCIO F.. State-of-the-art and perspectives in the use of biochar for electrochemical and electroanalytical applications. GREEN CHEMISTRY, v. 23, n. 15, . (19/01844-4, 17/21097-3, 13/22127-2, 19/00473-2)
VON ZUBEN, THEODORA WROBEL; KALINKE, CRISTIANE; JANEGITZ, BRUNO CAMPOS; SALLES JR, AIRTON GONCALVES; BONACIN, JULIANO ALVES. 3D-printed amperometric sensor for the detection of ethanol in saliva. Electroanalysis, v. N/A, p. 11-pg., . (21/07989-4, 19/21506-6, 13/22127-2, 19/00473-2)
DE SOUZA, DANIEL CARDOSO; ORZARI, LUIZ OTAVIO; DE OLIVEIRA, PAULO ROBERTO; KALINKE, CRISTIANE; BONACIN, JULIANO ALVES; MALASPINA, OSMAR; FERREIRA NOCELLI, ROBERTA CORNELIO; JANEGITZ, BRUNO CAMPOS. Electrochemical Sensor Based on Beeswax and Carbon Black Thin Biofilms for Determination of Paraquat in Apis mellifera Honey. FOOD ANALYTICAL METHODS, v. 14, n. 3, p. 606-615, . (19/01844-4, 19/00473-2, 13/22127-2, 17/21097-3)

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