Scholarship 19/04981-2 - Biologia molecular, Embriogênese animal - BV FAPESP
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Effects of extracellular vesicle supplementation during bovine embryo cleavage

Grant number: 19/04981-2
Support Opportunities:Scholarships in Brazil - Master
Start date until: April 01, 2019
End date until: March 31, 2021
Field of knowledge:Agronomical Sciences - Veterinary Medicine - Animal Reproduction
Principal Investigator:Juliano Coelho da Silveira
Grantee:Rosane Mazzarella
Host Institution: Faculdade de Zootecnia e Engenharia de Alimentos (FZEA). Universidade de São Paulo (USP). Pirassununga , SP, Brazil
Associated research grant:14/22887-0 - Cell-secreted vesicles containing miRNAs modulate epigenetic changes during in vitro culture of bovine gametes and embryos, AP.JP

Abstract

The use of assisted reproduction techniques is an alternative to solve reproductive problems in domestic species and in humans. However, there is concern that the in vitro culture of gametes and embryos can generate epigenetic changes which may lead to increased pre-disposition to cardiac, reproductive or tumor development in adult life. Epigenetic changes are regulated by DNA methylation, post-translational modifications of histones or by microRNAs (miRNA). The miRNAs are non-coding RNAs that regulate gene expression during different processes of tissue differentiation. Recently, vesicles secreted by cells, called exosomes and microvesicles, were found in different body fluids containing bioactive material including miRNAs. These extracellular vesicles (EVs) are considered a new class of cellular communication with possible implications in different physiological processes. However, its role in the acquisition of gametes' competence and in embryonic development is still unknown. The hypothesis that will be tested is that small EVs secreted by oviduct cells are able to alter oocyte fertilization in vitro and the development of bovine embryos produced in vitro. The objective is to evaluate the effects of supplementation of small EVs from oviduct cells in the fertilization of cumulus oocyte complexes (CCOs) and the in vitro embryo development of cattle. For this, EVs will be isolated from oviduct and oviduct conditioned medium and will be characterized. It is expected to determine the role of EVs supplementation during maturation and early embryonic development, which may influence the current in vitro production system. The results obtained in this project will allow the development of protocols more similar to the physiological condition for the in vitro production of embryos, with great impact in the assisted reproduction industry in cattle and in humans. (AU)

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Scientific publications (4)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
HAMDI, MERIEM; CANON-BELTRAN, KARINA; MAZZARELLA, ROSANE; CAJAS, YULIA N.; LEAL, CLAUDIA L. V.; GUTIERREZ-ADAN, ALFONSO; GONZALEZ, ENCINA M.; DA SILVEIRA, JULIANO C.; RIZOS, DIMITRIOS. Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle. FASEB JOURNAL, v. 35, n. 12, . (17/20339-3, 19/04981-2, 15/21829-9, 14/22887-0)
MAZZARELLA, ROSANE; BASTOS, NATALIA MARINS; BRIDI, ALESSANDRA; DEL COLLADO, MAITE; ANDRADE, GABRIELLA MAMEDE; PINZON, JORGE; PRADO, CIBELE MARIA; SILVA, LUCIANO ANDRADE; MEIRELLES, FLAVIO VIEIRA; PUGLIESI, GUILHERME; et al. Changes in Oviductal Cells and Small Extracellular Vesicles miRNAs in Pregnant Cows. FRONTIERS IN VETERINARY SCIENCE, v. 8, . (15/21829-9, 19/04981-2, 14/22887-0)
VERDE LEAL, CLAUDIA LIMA; CANON-BELTRAN, KARINA; CAJAS, YULIA N.; HAMDI, MERIEM; YARYES, ARACELLI; MILLAN DE LA BLANCA, MARIA GEMMA; BELTRAN-BRENA, PAULA; MAZZARELLA, ROSANE; DA SILVEIRA, JULIANO COELHO; GUTIERREZ-ADAN, ALFONSO; et al. Extracellular vesicles from oviductal and uterine fluids supplementation in sequential in vitro culture improves bovine embryo quality. JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY, v. 13, n. 1, p. 20-pg., . (17/20339-3, 19/04981-2, 15/21829-9, 14/22887-0)
P.C.S. NUNES; S.M. SILVA; R. MAZZARELLA; C.G. LIMA; J.C. SILVEIRA; D.C.A. LEITE-DELLOVA. [Vesículas extracelulares pequenas no soro de cães com sobrepeso e obesos, antes e depois da perda de peso: observações preliminares]. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 75, n. 1, p. 147-152, . (15/21829-9, 18/26547-0, 19/04981-2, 14/22887-0)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
MAZZARELLA, Rosane. Pregnancy effects in the bovine oviduct during early embryonic development in vivo: modulation of the microRNA profile in small extracellular vesicles of the oviductal fluid and oviductal epithelial cells. 2021. Master's Dissertation - Universidade de São Paulo (USP). Faculdade de Zootecnica e Engenharia de Alimentos (FZE/BT) Pirassununga.

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