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Effect of the combination of treatments with casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate on demineralization and enamel remineralization: in vitro studies

Grant number: 18/24761-4
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): April 01, 2019
Effective date (End): February 29, 2020
Field of knowledge:Health Sciences - Dentistry - Pediatric Dentistry
Principal Investigator:Marcelle Danelon
Grantee:Francyenne Maira Castro Gonçalves
Host Institution: Faculdade de Odontologia (FOA). Universidade Estadual Paulista (UNESP). Campus de Araçatuba. Araçatuba , SP, Brazil


The purpose of this study was to evaluate the association of treatments with fluoridated dentifrices and supplemented with sodium trimetaphosphate (TMP) and amorphous calcium phosphate casein (CPP-ACP) phosphopeptide (MI Paste Plus®) in reducing demineralization and promoting remineralization, respectively, of dental enamel. Bovine enamel blocks (12/group) will be selected through the initial surface hardness (SH) and divided into 6 experimental groups: 1) Toothpaste without F (Placebo); 2) Toothpaste with 1100 ppm F (1100F), 3) Toothpaste with 1100 ppm F + MI Paste Plus® (1100F/MI Paste Plus®), 4) Toothpaste with 1100 ppm F + 3% TMP (1100F/TMP), 5) MI Paste Plus® and 6) Toothpaste with 1100 ppm F + 3% TMP + MI Paste Plus® (1100F/TMP/MI Paste Plus®). For Demineralization Subproject 1 (DES>RE), the blocks will be subjected to 5 pH cycling for 7 days. For Remineralization Subproject 2 (RE>DES), the blocks will be selected by post-lesion surface hardness of artificial caries (SH1) and subjected to 6 pH cycling for 6 days. After the pH cycling, the final surface hardness (SH2) will be determined for the calculation of the percentage of surface hardness loss (%SH) and surface hardness recovery (%SHR), integrated loss of subsurface hardness (delta KHN), mineral content of enamel (, concentration of fluoride (F) calcium (Ca) and phosphorus (P) and analysis of the morphology and surface chemical composition by Scanning Electron Microscopy and X-ray Dispersive Energy enamel. The data will be submitted to the normality and homogeneity tests to use the statistical test most appropriate to the data distribution. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
CASTRO GONCALVES, FRANCYENNE MAIRA; BOTAZZO DELBEM, ALBERTO CARLOS; GOMES, LEONARDO FERNANDES; EMERENCIANO, NAYARA GONCALVES; PESSAN, JULIANO PELIM; AZEVEDO ROMERO, GIOVANA DORNELAS; CANNON, MARK L.; DANELON, MARCELLE. Effect of fluoride, casein phosphopeptide-amorphous calcium phosphate and sodium trimetaphosphate combination treatment on the remineralization of caries lesions: An in vitro study. ARCHIVES OF ORAL BIOLOGY, v. 122, . (20/03506-6, 17/21018-6, 18/24761-4)
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
GONÇALVES, Francyenne Maira Castro. Effect of the combination of treatments with casein phosphopeptide- amorphous calcium phosphate and sodium trimetaphosphate on desmineralization and enamel remineralization: in vitro studies. 2020. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Odontologia. Araçatuba Araçatuba.

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