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Application of the antimicrobial peptide Ctx(Ile21)-Ha and its conjugates in different formulations as a nutritional additive in laying hens

Grant number: 18/25707-3
Support type:Scholarships in Brazil - Master
Effective date (Start): March 01, 2019
Effective date (End): February 28, 2021
Field of knowledge:Agronomical Sciences - Animal Husbandry - Animal Nutrition and Feeding
Principal researcher:Eduardo Festozo Vicente
Grantee:Cesar Augusto Roque Borda
Home Institution: Faculdade de Ciências e Engenharia. Universidade Estadual Paulista (UNESP). Campus de Tupã. Tupã , SP, Brazil
Associated research grant:16/00446-7 - Application of antimicrobial peptides as new additives in animal nutrition, AP.JP

Abstract

Nowadays, markets are increasingly demanding more attention and requirements to the quality and safety of the food produced, also for the type of additives added for the animal feeding. In this way, the search for new compounds that can increase and improve productivity or reduce chemical residues in products of animal origin becomes relevant and demanded. In parallel, the constant use of synthetic antibiotics as growth promoters causes a high bacterial resistance in laying hens, as well as traces and residues in their products, which can affect animal health and, consequently, the human health. In this sense, the antimicrobial peptide Ctx(Ile21)-Ha, extracted and isolated from the Brazilian cerrado frog Hypsiboas albopunctatus, has been studied extensively by the present research group and has been shown, in previous studies, to be very active and promising molecule in the control of pathogenic bacteria in laying hens. Thus, this project aims the synthesis, purification and characterization of the peptide Ctx(Ile21)-Ha, in addition to its conjugation with chitosan, very known as natural antimicrobial molecules, for the formation of the conjugate here called peptosan. After this step, the new molecules will be formulated into microparticles with two different coating types (HPMC phthalate and acetate/succinate) for direct release into the bowel of the laying hens. By means of microbiological techniques, the bacterial colonies of Salmonella ssp. will be evaluated in the cecal and intestinal contents as a whole, after the addition of the molecules. In addition, it is expected to carry out the analysis of zootechnical performance (average daily intake, daily weight gain, weight at 28 days) and total digestibility. Thus, it is expected that the number of CFUs of the pathogenic bacteria decreases after the administration of peptosanes and if the registered zootechnical indexes are positive, this study can enabling the peptide Ctx(Ile21)-Ha and its conjugates (peptosanas) as a new nutritional additive in laying hens. (AU)

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Scientific publications (5)
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
SILVEIRA, RAIZA F.; ROQUE-BORDA, CESAR A.; VICENTE, EDUARDO F. Antimicrobial peptides as a feed additive alternative to animal production, food safety and public health implications: An overview. ANIMAL NUTRITION, v. 7, n. 3, p. 896-904, SEP 2021. Web of Science Citations: 6.
ROQUE-BORDA, CESAR AUGUSTO; LEITE SILVA, HANYENY RAIELY; CRUSCA JUNIOR, EDSON; SERAFIM, JESSICA APARECIDA; MENEGUIN, ANDREIA BAGLIOTTI; CHORILLI, MARLUS; MACEDO, WAGNER COSTA; TEIXEIRA, SILVIO RAINHO; LOPES GUASTALLI, ELISABETE APARECIDA; SOARES, NILCE MARIA; BLAIR, JESSICA M. A.; PIKRAMENOU, ZOE; VICENTE, EDUARDO FESTOZO. Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile(21))-Ha antimicrobial peptide application as a feed additive. International Journal of Biological Macromolecules, v. 183, p. 1236-1247, JUL 31 2021. Web of Science Citations: 4.
ROQUE-BORDA, CESAR AUGUSTO; KULUS, DARIUSZ; VACARO DE SOUZA, ANGELA; KAVIANI, BEHZAD; VICENTE, EDUARDO FESTOZO. Cryopreservation of Agronomic Plant Germplasm Using Vitrification-Based Methods: An Overview of Selected Case Studies. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, v. 22, n. 11 JUN 2021. Web of Science Citations: 0.
ROQUE-BORDA, CESAR AUGUSTO; PEREIRA, LARISSA PIRES; GUASTALLI, ELISABETE APARECIDA LOPES; SOARES, NILCE MARIA; MAC-LEAN, PRISCILLA AYLEEN BUSTOS; SALGADO, DOUGLAS D'ALESSANDRO; MENEGUIN, ANDREIA BAGLIOTTI; CHORILLI, MARLUS; VICENTE, EDUARDO FESTOZO. HPMCP-Coated Microcapsules Containing the Ctx(Ile(21))-Ha Antimicrobial Peptide Reduce the Mortality Rate Caused by Resistant Salmonella Enteritidis in Laying Hens. ANTIBIOTICS-BASEL, v. 10, n. 6 JUN 2021. Web of Science Citations: 0.
ROQUE-BORDA, CESAR AUGUSTO; DA SILVA, PATRICIA BENTO; RODRIGUES, MOSAR CORREA; AZEVEDO, RICARDO BENTES; DI FILIPPO, LEONARDO; DUARTE, JONATAS L.; CHORILLI, MARLUS; VICENTE, EDUARDO FESTOZO; PAVAN, FERNANDO ROGERIO. Challenge in the Discovery of New Drugs: Antimicrobial Peptides against WHO-List of Critical and High-Priority Bacteria. PHARMACEUTICS, v. 13, n. 6 JUN 2021. Web of Science Citations: 1.
Academic Publications
(References retrieved automatically from State of São Paulo Research Institutions)
BORDA, Cesar Augusto Roque. . 2021. Master's Dissertation - Universidade Estadual Paulista (Unesp). Faculdade de Ciências Agrárias e Veterinárias. Jaboticabal Jaboticabal.

Please report errors in scientific publications list by writing to: cdi@fapesp.br.