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Effect of miR-135b from mesenchymal stem cells on the production of extracellular vesicles with angiogenic factors

Grant number: 18/21072-3
Support Opportunities:Scholarships in Brazil - Master
Effective date (Start): March 01, 2019
Effective date (End): September 30, 2020
Field of knowledge:Biological Sciences - Biochemistry - Molecular Biology
Principal Investigator:Sang Won Han
Grantee:Juliana Maíra Freitas Vieira
Host Institution: Escola Paulista de Medicina (EPM). Universidade Federal de São Paulo (UNIFESP). Campus São Paulo. São Paulo , SP, Brazil

Abstract

The critical limb ischemia (CLI) is characterized by the narrowing of arteries from lower limbs. It is being estimated that 25% of patients diagnosticated with CLI will die and others 30% will receive amputation within a year. The CLI available treatments are limited due co-morbidities and advanced age of patients, thus, it is necessary to develop new alternative therapies. Among these, the angiogenic therapy is promising, by restoring the oxygen and nutrients supply in the injured tissue. Angiogenesis undergoes the microRNAs (miRs) control and, amidst them, it is possible to highlight the miR-135b because of its influence in the HIF-FIH signal pathway, an important axis in this biological process. However, the miRs are fragile and easy degraded, therefore, it is necessary a way of administration that allows these molecules to reach their target and prolong their duration in the organism. The extracellular vesicles (EVs) from mesenchymal stem cells (MSCs) are potential vehicles for miRs since their biocompatibility, their capacity to pass the plasma membrane and their bioavailability, besides, they are naturally load with angiogenic factors. Hence, the present project raises the questions: Will the modified MSCs, with miR-135b, produce EVs with better angiogenic potential? And what will be the cargo difference of these EVs compared with non-modified MSCs? The obtained answers in this project will improve the understanding of miRs and EVs roles in the angiogenesis and it will seek a new resource for CLI angiogenic therapy. (AU)

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Scientific publications
(References retrieved automatically from Web of Science and SciELO through information on FAPESP grants and their corresponding numbers as mentioned in the publications by the authors)
FREITAS VIEIRA, JULIANA MAIRA; ZAMPRONI, LAURA NICOLETI; WENDT, CAMILA H. C.; DE MIRANDA, KILDARE ROCHA; LINDOSO, RAFAEL SOARES; HAN, SANG WON. Overexpression of mir-135b and mir-210 in mesenchymal stromal cells for the enrichment of extracellular vesicles with angiogenic factors. PLoS One, v. 17, n. 8, p. 19-pg., . (18/21072-3, 15/20206-8)

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